Multilocus genotyping for classification and genetic structuring of Lactobacillus casei: insights from source and geographical origin
Tynybayeva I. Tekebayeva Z. Bissenova G. Mynbayeva M. Sarmurzina Z.
March 2024Springer Science and Business Media B.V.
World Journal of Microbiology and Biotechnology
2024#40Issue 3
The aim of the study in this article is to systematise the newly introduced strains of Lactobacillus based on determining the nucleotide sequence of a particular set of their genes (loci). The primary approach employed to address this issue involves conducting a laboratory experiment. During this experiment, a thorough examination was carried out on a set of organic compounds consisting of small DNA elements from the Lactobacillus genus. The Multilocus genotyping method served as the central technique, complemented by additional molecular-biological and population methods. These additional methods were utilized to determine the extent of phylogenetic similarity among pure cultures of Lactobacillus and to classify them accordingly. The article presents the gene isolates that were used for Multilocus typing; the number of L. casei isolates suitable for Multilocus genotyping was revealed; the gene alleles that allowed classifying L. casei isolates into five sequencing types were revealed; the effectiveness of genetic typing method for Multilocus sequencing was substantiated. The article is of practical value for microbiologists and geneticists in the field of molecular biology, as well as for technologists in the food industry. With the development of applied methods in genetic systematics, it has become possible to study pure culture of Lactobacillus species. The application of modern methods of genotypic classification of Lactobacillus species will make it possible to increase the efficiency of using better and safer products in the food industry and medicine.
L. casei , Lactobacillus , Nucleotide sequence , Phenotypic taxonomy , Sequencing , Systematics
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Laboratory of Genetics and Biochemistry of Microorganisms, Republican Collection of Microorganisms, Astana, 010000, Kazakhstan
Laboratory of Microbiology, Republican Collection of Microorganisms, Astana, 010000, Kazakhstan
Laboratory of Biotechnology, Astana Bioscience Business Centre LLP, Astana, 010000, Kazakhstan
Center for Study of Antibiotics and Secondary Metabolites, Republican Collection of Microorganisms, Astana, 010000, Kazakhstan
Laboratory of Genetics and Biochemistry of Microorganisms
Laboratory of Microbiology
Laboratory of Biotechnology
Center for Study of Antibiotics and Secondary Metabolites
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