Electroactive Microbial Consortium of Bacillus, Lysinibacillus, and Lactococcus for Enhanced Wastewater Treatment and Bioelectricity Generation
Temirbekova A. Tekebayeva Z. Mkilima T. Kulzhanova K. Nurbekova Z. Temirkhanov A. Meiramkulova K. Bekshin Z. Abzhalelov A.
January 2026Multidisciplinary Digital Publishing Institute (MDPI)
Biology
2026#15Issue 2
Microbial fuel cell (MFC) technology represents a promising bioelectrochemical approach for the simultaneous generation of electricity and treatment of high-strength wastewater. However, the performance of MFCs strongly depends on the metabolic potential and synergistic interactions of the inoculated microbial community. This study evaluated the electrochemical activity and COD removal efficiency of three individual bacterial strains, Lysinibacillus sphericus A1, Bacillus cereus A2 and Lactococcus lactis A4, compared with a developed consortium under long-term operation using poultry slaughterhouse wastewater as a substrate. All inocula were tested in dual-chamber MFCs for 30 days, and performance indicators included power output, voltage, and removal of chemical oxygen demand (COD). The consortium showed the highest power of 170 mW/m2 and the optimal voltage–current ratio at a current of 900 mA/m2 and 245 mV under decreasing external resistance from 1000 to 50 Ω. The highest COD removal (84.4%) was also recorded, surpassing all pure cultures and demonstrating a significant improvement compared with B. cereus A2 and L. lactis A4. Meanwhile, the lowest power of 52 mA/m2 was recorded during testing of L. lactis A4, at 650 mA/m2 and 120 mV. Compared with single cultures, the consortium produced approximately 15% higher power density than L. sphericus A1, about 29% higher than B. cereus A2, and more than threefold higher than L. lactis A4. This study highlights the potential of a consortium as an efficient biocatalyst for MFC-mediated wastewater treatment and suggests that selecting complementary strains with diverse metabolic functions can substantially improve system performance.
bioelectrochemical systems , chemical oxygen demand removal , electrogenic performance optimisation , poultry wastewater biodegradation , synergistic microbial interactions
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Republican Collection of Microorganisms, Astana, 010000, Kazakhstan
Department of General Biology and Genomics, L.N. Gumilyov Eurasian National University, Astana, 010000, Kazakhstan
Department of Environmental Engineering and Management, The University of Dodoma, 1 Benjamin Mkapa Road, Iyumbu, Dodoma, 41218, Tanzania
Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian National University, Astana, 010000, Kazakhstan
Department of Management and Engineering in the Field of Environmental Protection, L.N. Gumilyov Eurasian National University, Astana, 010000, Kazakhstan
Republican Collection of Microorganisms
Department of General Biology and Genomics
Department of Environmental Engineering and Management
Department of Biotechnology and Microbiology
Department of Management and Engineering in the Field of Environmental Protection
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