Antioxidant, Anti-Tumour, and Anticoagulant Activities of Polysaccharide from Calocybe indica (APK2)

Nataraj A. Govindan S. Ramani P. Subbaiah K.A. Sathianarayanan S. Venkidasamy B. Thiruvengadam M. Rebezov M. Shariati M.A. Lorenzo J.M. Pateiro M.
September 2022 MDPI

Antioxidants
2022 #11 Issue 9

The initial structural features and in vitro biological study of crude polysaccharides from Calocybe indica (CICP) extracted by hot water followed by ethanol precipitation was investigated. High-performance gel permeation chromatography, HPLC-DAD, UV, IR and NMR spectroscopy, X-ray diffraction, scanning electron microscopy, and Congo red methods were used to determine structural features. The results revealed that CICP is a hetero-polysaccharide with a molecular weight of 9.371 × 10⁴ Da and 2.457 × 10³ Da which is composed of xylose, mannose, fucose, rhamnose, arabinose, galactose, and glucose. The antioxidant activity of CICP was evaluated using radical scavenging activity (three methods), reducing ability (three methods), metal chelating activity, and lipid peroxidation inhibition activity (two methods). It was found that the antioxidant capacity is concentration-dependent and EC₅₀ values were found to be 1.99–3.82 mg/mL (radical scavenging activities), 0.78–2.78 mg/mL (reducing ability), 4.11 mg/mL (metal chelating activity), and 0.56–4.18 mg/mL (lipid peroxidation inhibition activity). In vitro anticoagulant assay revealed that CICP could prolong activated partial thromboplastin time (APTT), thrombin time (TT), but not prothrombin time (PT). CICP exhibited antiproliferative activity on HeLa, PC3, HT29, HepG2, and Jurkat cell lines with IC₅₀ (μg/mL) values of 148.40, 143.60,151.00, 168.30, and 156.30, respectively. The above findings suggested that CICP could be considered a natural antioxidant and cancer preventative.

anticoagulant , antioxidant , antiproliferative activity , Calocybe indica , edible mushroom , polysaccharides

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Department of Biochemistry, School of Biosciences, Periyar University, Salem, 636011, India
Dhanvanthri Laboratory, Department of Sciences, Amrita School of Physical Sciences, Amrita Vishwa Vidyapeetham, Coimbatore, 641112, India
Center of Excellence in Advanced Materials Green Technologies (CoE–AMGT), Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, 641112, India
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, 641003, India
Faculty of Pharmacy, Karpagam Academy of Higher Education, Coimbatore, 641021, India
Department of Oral Maxillofacial Surgery, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, India
Department of Crop Science, College of Sanghuh Life Science, Konkuk University, Seoul, 05029, South Korea
Department of Scientific Research, V. M. Gorbatov Federal Research Center for Food Systems, 26 Talalikhin st, Moscow, 109316, Russian Federation
Biophotonics center, Prokhorov General Physics Institute of the Russian Academy of Science, 38 Vavilov st., Moscow, 119991, Russian Federation
Semey Branch of the Institute, Kazakh Research Institute of Processing and Food Industry, 238«G» Gagarin Ave, Almaty, 050060, Kazakhstan
Centro Tecnológico de la Carne de Galicia, Parque Tecnológico de Galicia, Avd. Galicia No. 4, San Cibrao das Viñas, Ourense, 32900, Spain
Facultade de Ciencias, Universidade de Vigo, Área de Tecnoloxía dos Alimentos, Ourense, 32004, Spain

Department of Biochemistry
Dhanvanthri Laboratory
Center of Excellence in Advanced Materials Green Technologies (CoE–AMGT)
Department of Plant Pathology
Faculty of Pharmacy
Department of Oral Maxillofacial Surgery
Department of Crop Science
Department of Scientific Research
Biophotonics center
Semey Branch of the Institute
Centro Tecnológico de la Carne de Galicia
Facultade de Ciencias

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