Effect of Lactobacterin-TK2 and Multispecies probiotics on calf health during suckling

Özkaya S. Işık A.R. Boranbayeva T. Karahan A.G. Tulemissova Z. Myktybayeva R. Sansyzbai A. Şanlı E.R.
17 December 2025 Hellenic Veterinary Medical Society

Journal of the Hellenic Veterinary Medical Society
2025 #76 Issue 4 9877 - 9890 pp.

We hypothesised that supplementing probiotics to milk would improve calves’ intestinal microbiota, support the immune system and antioxidant defence mechanism, increase their live weight, and raise healthy calves. The present study aimed to investigate the effects of two probiotic mixtures (Lactobacterin-TK² and Hypro) containing beneficial microorganisms from different sources on the immune system and growth of calves by reducing oxidative stress during the milk-suckling period. Eighteen newborn calves (n=6 in each group) were randomly selected and divided into three groups. Groups were formed as the control group (G1; without probiotics), Lactobacterin-TK² (G2), and Hypro probiotic (G3). The administration of Lactobacterin-TK² and Hypro probiotics caused an increase in live weight (LW, P=0.04) and daily live weight (DLW, P=0.04) and reduced the number of days with diarrhoea compared to the control group. Hypro reduced the total coliform counts (P<0.05) and increased yeast counts (P<0.05) in faecal samples on 14-day-age. No adverse effects of probiotics were determined on blood biochemistry. No significant changes were determined in the oxidative and antioxidative stress parameters, except for Paraoxonase 1 (PON 1) (P<0.05). IgA, IgE, and IgG levels in the probiotic groups showed similarities with those of the control group. The findings showed that Lactobacterin-TK² and Hypro probiotics can be used to supplement the milk of calves to provide better growth during the suckling period and to raise healthy calves by reducing the effect of oxidative stress.

calf , growth , health , Probiotics

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Isparta University of Applied Sciences, Agricultural Faculty, Department of Animal Science, Isparta, 32200, Turkey
Kazakh National Agrarian Research University, Faculty of Bioresources and Technology, Department of Technology and Safety of Food Products, Almaty, Kazakhstan
Suleyman Demirel University, Faculty of Engineering, Department of Food Engineering, Isparta, 32200, Turkey
Kazakh National Agrarian Research University, Faculty of Veterinary, Department of Biological Safety, Almaty, Kazakhstan
Kazakh National Agrarian Research University, Faculty of Veterinary, Department of Microbiology and Virology, Almaty, Kazakhstan

Isparta University of Applied Sciences
Kazakh National Agrarian Research University
Suleyman Demirel University
Kazakh National Agrarian Research University
Kazakh National Agrarian Research University

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