Development of Alginate Composite Microparticles for Encapsulation of Bifidobacterium animalis subsp. lactis
Vinceković M. Živković L. Turkeyeva E. Mutaliyeva B. Madybekova G. Šegota S. Šijaković Vujičić N. Pustak A. Jurkin T. Kiš M. Kajić S.
November 2024Multidisciplinary Digital Publishing Institute (MDPI)
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2024#10Issue 11
The probiotic bacterium Bifidobacterium animalis subsp. lactis BB-12 (BB-12) was encapsulated in two composites, alginate/agar and alginate/agar/casein. The network structure and physicochemical properties of these composites are influenced by complex interactions, including hydrogen bonding, electrostatic forces between biopolymers, calcium ions, and the encapsulated bacteria. The composites demonstrated a granular surface, with the granules being spatially oriented on the alginate/agar/BB-12 surface and linearly oriented on the alginate/agar/casein/BB-12 surface. They possess a highly organized microparticle structure and exhibit viscoelastic solid-like behavior. The alginate/agar/BB-12 composite showed higher storage modulus, shear stress, and shear strain values, indicating enhanced stability in various physical environments. Both composites displayed good thermal stability, aligning with their rheological properties, confirming their well-ordered structures. Despite differences in composite structures, the release mechanism of bacteria is governed by Fickian diffusion through the composite matrix. Based on physicochemical properties, the alginate/agar/casein composite is recommended for dairy product fermentation, while the alginate/agar composite seems more suitable for oral use. These findings provide new insights into the interactions between bacterial cultures and alginate composite ingredients.
agar , alginate composite , Bifidobacterium animalis subsp. lactis BB-12 , casein , encapsulation
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Division of Agroecology, Department of Chemistry, University of Zagreb, Faculty of Agriculture, Svetošimunska 25, Zagreb, 10000, Croatia
Biotechnology Department, M. Auezov South-Kazakhstan University, Shymkent, Tauke-Khan, 160000, Kazakhstan
Chemistry Department, O. Zhanibekov South-Kazakhstan Pedagogical University, 13, Shymkent, Baitursynov Street, 160000, Kazakhstan
Laboratory for Biocolloids and Surface Chemistry, Ruđer Bošković Institute, Bijenička c. 54, Zagreb, 10000, Croatia
Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička c. 54, Zagreb, 10000, Croatia
Radiation Chemistry and Dosimetry Laboratory, Ruđer Bošković Institute, Bijenička c. 54, Zagreb, 10000, Croatia
Faculty of Veterinary Medicine, University of Zagreb, Ul. Vjekoslava Heinzela 55, Zagreb, 10000, Croatia
Division of Agroecology, Department of Microbiology, University of Zagreb, Faculty of Agriculture, Zagreb, 10000, Croatia
Division of Agroecology
Biotechnology Department
Chemistry Department
Laboratory for Biocolloids and Surface Chemistry
Division of Organic Chemistry and Biochemistry
Radiation Chemistry and Dosimetry Laboratory
Faculty of Veterinary Medicine
Division of Agroecology
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