The effect of camel milk on house dust mite allergen induced asthma model in BALB/C mice

Rakhmatulina A. Kenenbay S. Abuova A. Kizatova M. Ibraikhan A. Makhmudov F. Mukashev A. Aitbaeva A. Abilkaiyr Z. Ibadullayeva G. Chomanov U. Murzabulatov A. Azimova S. Kulpiisova A. Bayantassova S. Aralbayev N. Imanbayeva N. Dikhanbayeva F. Burambayeva N. Smagulova N. Issimov A. White P.
June 2025 Public Library of Science

PLOS ONE
2025 #20 Issue 6 JUNE

Camel milk has demonstrated robust immunomodulatory and anti-inflammatory properties in various clinical and experimental studies. However, no previous studies have characterized the cellular immunological effects of camel milk in the context of allergic asthma. Therefore, the present work aimed to evaluate the protective effects of camel milk in house dust mite induced asthma in mice, which emulate human pulmonary inflammation. Female BALB/c mice aged 8- to 10-week-old were intranasally sensitized with vehicle or HDM in 2.5 µl (5 µg) per nostril, 5 days a week for 3 weeks. On day 22, mice received an HDM challenge by a large volume but low dose into the lung (5 µg in 50µl) using intranasal inoculation. Using oral gavage technique, CM/ HDM group mice received 0.5 ml of camel milk or vehicle five times a week, starting a day prior to sensitization. On day 23 following HDM challenge, mice were exposed to serial challenges with 10, 20, 40 and 100 mg/ml aerosolized methacholine to measure lung dynamics. Furthermore, BALF and whole lung samples were harvested to examine pulmonary inflammation. Camel milk effectively inhibited both HDM-induced infiltration of eosinophils and AHR. In addition to this, camel milk downregulates the number of pulmonary Th2 and Th17 cells and suppressed CCL17 expression in whole lung homogenates. Furthermore, camel milk reduced HDM-induced IL-4 and IL-13 expression following in vitro restimulation of pulmonary T cell subsets. Additionally, camel milk suppressed total concentrations of IL-5 and IL-13 in the lung. These results corroborate the asthma-preventive potential of camel milk and highlight the significance of diminished local concentrations of Th2- associated cytokines. In the present study, the observed downregulation of asthma progression by camel milk suggests its potential health benefits; however, further experimental and controlled clinical trials are needed before it can be considered a supplementary approach for allergic asthma management.

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Department of Mechanical Engineering and Robotics, Joldasbekov Institute of Mechanics and Engineering, Almaty, Kazakhstan
Department of Food Technology, Food Safety and Quality, Almaty Technological University, Almaty, Kazakhstan
Department of Food Technology, International Engineering Technological University, Almaty, Kazakhstan
Department of Pharmaceutical Technology, S.D. Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan
Kazakh-German Research Institute “Qymyz”, Almaty, Kazakhstan
Department of Food Technology and Safety of Food Products, M. Auezov South – Kazakhstan University, Shymkent, Kazakhstan
Department of Mechanical Engineering, Satbayev University, Almaty, Kazakhstan
Scientific Consultant, “Paritet” LLP, Almaty, Kazakhstan
Department of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kazakhstan
Department of Veterinary Medicine, Zhangir Khan West Kazakhstan Agrarian Technical University, Oral, Kazakhstan
Department of Zootechnology, Genetics and Breeding, Toraighyrov University, Pavlodar, Kazakhstan
Department of Biology, Zhubanov Aktobe Regional University, Aktobe, Kazakhstan
Sydney School of Veterinary Science, Faculty of Science, University of Sydney, Sydney, Australia

Department of Mechanical Engineering and Robotics
Department of Food Technology
Department of Food Technology
Department of Pharmaceutical Technology
Kazakh-German Research Institute “Qymyz”
Department of Food Technology and Safety of Food Products
Department of Mechanical Engineering
Scientific Consultant
Department of Veterinary Medicine
Department of Veterinary Medicine
Department of Zootechnology
Department of Biology
Sydney School of Veterinary Science

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