Identification and characterization of salt stress-responsive NHX gene family in chickpea
Parveen K. Saddique M.A.B. Rehman S.U. Ali Z. Aziz I. Shamsi I.H. Muneer M.A.
December 2023Elsevier B.V.
Plant Stress
2023#10
Chickpea (Cicer arietinum L.) is an important legume crop, primarily grown in the arid and semi-arid regions. It is a valuable protein source and rich in minerals, especially calcium and phosphorus. Salinity stress adversely affects chickpea germination, vegetative growth, and reproductive activities. The Na+/H+ exchanger (NHX), a gene family of membrane transporters, is well recognized for inducing salt tolerance in plants. The NHXs assist in maintaining ion and pH homeostasis in cells by exchanging K+ or Na+ for H+. This study was designed to identify and characterize the NHX genes for salt stress responsiveness in chickpea. Eight NHX genes were identified in chickpea. The phylogenetic analysis represented the evolutionary relationship of Cicer arietinum NHXs (CarNHXs) with Glycine max, Medicago truncatula, Lotus japonicus, Phaseolus vulgaris, Lens culinaris, Vigna unguiculata, and Oryza sativa. The in-silico gene expression analysis indicated high expression of CarNHX3 (Ca_19073) and CarNHX7 (Ca_02050) under salt stress. The qRT-PCR further validated that the expression of CarNHX3 and CarNHX7 increased when plants were exposed to salt stress. This study provides a way forward to plan functional research to exploit CarNHX genes to enhance salt-tolerance in chickpea.
CarNHX3 , CarNHX7 , Gene expression , Na+/H+ exchanger , Phylogenetic analysis , qRT-PCR , Salt stress
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Institute of Plant Breeding and Biotechnology, MNS University of Agriculture Multan, Pakistan
SINO-PAK Joint Research Laboratory, Institute of Plant Breeding and Biotechnology, MNS University of Agriculture Multan, Pakistan
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan
Programs and Projects Department, Islamic Organization for Food Security, Mangilik Yel Ave. 55/21 AIFC, Unit 4, C4.2, Astana, Kazakhstan
Department of Plant Breeding and Genetics, Ghazi University, Dera Ghazi Khan, Pakistan
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China
International Magnesium Institute, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China
Institute of Plant Breeding and Biotechnology
SINO-PAK Joint Research Laboratory
Department of Plant Breeding and Genetics
Programs and Projects Department
Department of Plant Breeding and Genetics
Department of Agronomy
International Magnesium Institute
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