Equine lactoferrin: Antioxidant properties related to divalent metal chelation
Narmuratova Z. Hentati F. Girardet J.-M. Narmuratova M. Cakir-Kiefer C.
1 May 2022Academic Press
LWT
2022#161
Lactoferrin is a minor whey protein known for its multifunctionalities, particularly for its rule in regulation of immune system and for its antimicrobial activities. Although bovine lactoferrin has been extensively studied, very few information exists on lactoferrin from mares milk. This article studies the antioxidant properties via metal chelation of purified lactoferrin from Kazakhstan mares milk. Equine lactoferrin shows free radical-scavenger activity when tested with radical DPPH and ABTS and has reducing capacities testing by FRAP. Moreover, the ferrous(II) and copper(II) ion-chelating power is highlighted by chemical colorimetric tests. Finally, the newly introduced biophysics switchSENSE® technology allowed to measure in real time the molecular interactions between equine lactoferrin and divalent cations. The order of magnitude of the KD values was tens or so μM for the four metal ions tested: Zn2+ (KD = 23.9 ± 4.0 μM), Ca2+ (KD = 28.2 ± 4.4 μM), Cu2+ (KD = 43.5 ± 5.1 μM) and Fe2+ (KD = 54.1 ± 8.9 μM). According to these results, we can conclude that equine lactoferrin shows antioxidant activity by radical-scavenging mechanism, reducing capacities and divalent pro-oxidant metals chelation ability.
Antioxidant , Equine lactoferrin , Metal chelation , switchSENSE® technology
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Kazakh National University Al-Farabi, Faculty of Biology and Biotechnology, Almaty, 050040, Kazakhstan
Université de Lorraine, INRAE, URAFPA, Nancy, F-54000, France
Université de Lorraine, INRAE, IAM, UMR 1136, Nancy, F-54000, France
Kazakh National University Al-Farabi
Université de Lorraine
Université de Lorraine
10 лет помогаем публиковать статьи Международный издатель
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