Probiotic Characterization of Lactic Acid Bacteria Isolated from Traditional Fermented Camel and Mare’s Milk Products
Kudaibergenova A. Begdildayeva N. Amirkhanova A. Nurgazina A. Ospanova A. Kondybayev A. Jumagaziyeva A. Iskakbayeva Z. Jumabayeva S. Akhmetsadykova S.
September 2025Engineered Science Publisher
ES Food and Agroforestry
2025#21
This study evaluated the probiotic potential of lactic acid bacteria (LAB) strains isolated from traditional fermented camel milk products, koumiss and shubat, collected in Kazakhstan. A total of nine LAB isolates were assessed for key probiotic properties, including acid tolerance (pH 2.0–3.0), bile salt resistance (0.3%), phenol and pepsin tolerance, NaCl stress (6.5% and 10%), thermal adaptability (4°C, 37°C, 55°C), and adhesion to intestinal epithelial cells. All isolates demonstrated robust growth at 6.5% NaCl and optimal temperature of 37°C, while growth was significantly inhibited at 10% NaCl and extreme temperatures. Most strains showed moderate to high survival under acidic conditions, with viability decreasing over time. Pepsin and phenol tolerance varied among strains, with a general trend of reduced viability as exposure time or concentration increased. Antibiotic susceptibility testing revealed sensitivity to clinically relevant antibiotics such as gentamicin, erythromycin, and ampicillin, while intrinsic resistance to penicillin was noted. Adhesion to HT-29 intestinal epithelial cells confirmed their colonization potential. Overall, the LAB strains exhibited strong and diverse probiotic characteristics, comparable to or exceeding those reported in previous literature. These findings highlight the potential of these indigenous strains for the development of region-specific functional probiotics, with applications in human nutrition, gut health restoration, and livestock gastrointestinal support.
Acid tolerance , Antibiotic susceptibility , Bile salt resistance , Camel milk , Functional foods , HT-29 adhesion , Kazakhstan , Lactic acid bacteria , Probiotics
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Department of Biophysics, Biomedicine and Neuroscience, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Department of Biotechnology, LLP “Research and Production Enterprise Antigen, 4 Azerbayev Street, Almaty, 040905, Kazakhstan
School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty, 050012, Kazakhstan
Department of Biotechnology, M. Auezov South Kazakhstan University, 5 Tauke khan Avenue, Shymkent, 160012, Kazakhstan
Department of Chemical and Biochemical Engineering, Satbayev University, 22 Satpaev street, Almaty, 050013, Kazakhstan
JSC Scientific Center for Anti-Infectious Diseases, 75B Al-Farabi Avenue, Almaty, 050035, Kazakhstan
Camel Breeding Department, LLP Kazakh Research Institute for Livestock and Fodder Production, 51 Zhandosov Street, Almaty, 050035, Kazakhstan
Department of Biophysics
Department of Biotechnology
School of Pharmacy
Department of Biotechnology
Department of Chemical and Biochemical Engineering
JSC Scientific Center for Anti-Infectious Diseases
Camel Breeding Department
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