Growth and Metabolism of Lacticaseibacillus casei and Lactobacillus kefiri Isolated from Qymyz, a Traditional Fermented Central Asian Beverage
Kondybayev A. Konuspayeva G. Strub C. Loiseau G. Mestres C. Grabulos J. Manzano M. Akhmetsadykova S. Achir N.
August 2022MDPI
Fermentation
2022#8Issue 8
The growth characteristics of two strains of lactic acid bacteria (LAB), Lacticaseibacillus casei and Lactobacillus kefiri, isolated from qymyz, a traditional fermented mare milk beverage, were studied and modeled, including the effect of different carbohydrates, pH, and temperature. Along with population, substrates, and metabolites, lactic acid and ethanol were monitored by HPLC. Growth parameters were obtained from mono- and biphasic logistic growth models that fit the population evolution of L. casei and L. kefiri, respectively. The effect of temperature and pH on the growth rate was represented with the gamma concept model, while the effect of the limiting substrate was evaluated according to the Monod equation. Lastly, a simplified Luedeking and Piret equation was used to represent metabolite production. The optimum values of pH and temperature were 6.69 ± 0.20, 38.63 ± 0.32 °C, 5.93 ± 0.08, and 33.15 ± 0.53 °C, with growth rate values of 0.66 ± 0.01 h−1 and 0.29 ± 0.01 h−1 for L. casei and L. kefiri, respectively. L. casei had a homofermentative pathway, while L. kefiri was heterofermentative, with an ethanol production rate of 2.90 × 10−9 mg·CFU−1. The Monod model showed that L. casei had the lowest Ks value for lactose, while for L. kefiri, it was the highest among milk carbohydrates. These results show that the population of the two LAB strains and therefore the concentrations of acid and ethanol can be controlled by the fermentation conditions and that our model can help to significantly improve the production of qymyz.
lactic acid bacteria , mare milk , mathematical modeling , qymyz (koumiss)
Text of the article Перейти на текст статьи
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, 71 Al-Farabi Av., Almaty, 050040, Kazakhstan
Qualisud, Univ Montpellier, Avignon Université, CIRAD, Institut Agro, IRD, Université de La Réunion, Montpellier, F-97490, France
Antigen Co. Ltd, Almaty Region, 4 Azerbayev Str, Almaty, 040905, Kazakhstan
CIRAD, UMR Qualisud, Montpellier, F-34398, France
Faculty of Biology and Biotechnology
Qualisud
Antigen Co. Ltd
CIRAD
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026