Response Surface Methodology Optimization on Ultrasonic-Assisted Extraction of Chicory (Cichorium intybus L.) and Their UPLC-ESI-QTOF-MS Analysis, Antioxidant and Hepatoprotective Activity

Baiseitova A. Amzeyeva U. Tolepbergen A. Ulfanova Y. Shybyray Y. Dai L. Shang X. Shah A.B. Kim J.Y. Jenis J.
August 2025 John Wiley and Sons Inc

eFood
2025 #6 Issue 4

Chicory (Cichorium intybus L.) roots are commonly roasted and ground to produce a coffee alternative, valued for its robust, nutty taste and absence of caffeine. Historically, it has been utilized to facilitate digestion, serve as a diuretic, and promote liver function. This study employed a response surface methodology (RSM) for optimization the ultrasonic-assisted extraction of antioxidant composition from chicory. The optimal extraction conditions (temperature 60°C, extraction duration 4 h, and solid-to-solvent ratio of 1:20 g/mL) resulted in the maximum yield and antioxidant capacity. The metabolic content of chicory extract, prepared under optimal conditions, was found by UPLC-ESI-QTOF/MS, including aesculetin, caftaric acid, caffeoylmalic acid, scopoletin, and chicoric acid. Moreover, chicory extracts derived under identical conditions in two distinct solvents (96% and 50% ethanol) demonstrated vitality and hepatoprotective action in HL-7702 cells. This study may offer a perspective for acquiring potential extracts of chicory as a functional food.

antioxidant , Chicory (Cichorium intybus L.) , hepatoprotective , response surface methodology (RSM) , UPLC-ESI-QTOF/MS

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Research Institute for Natural Products & Technology, Almaty, Kazakhstan
Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University, Jinju, South Korea
The Research Center for Medicinal Plants, Al-Farabi Kazakh National University, Almaty, Kazakhstan
Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, China
Kazakh-China Joint Research Center for Natural Veterinary Drug, Lanzhou, China
College of Pharmacy, Korea University, Sejong, South Korea
Department of Pharmaceutical Engineering, IALS, Gyeongsang National University, Jinju, South Korea

Research Institute for Natural Products & Technology
Division of Applied Life Science (BK21 plus)
The Research Center for Medicinal Plants
Lanzhou Institute of Husbandry and Pharmaceutical Sciences
Kazakh-China Joint Research Center for Natural Veterinary Drug
College of Pharmacy
Department of Pharmaceutical Engineering

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