Serum Metabolomics Uncovers Immune and Lipid Pathway Alterations in Lambs Supplemented with Novel LAB-Bifidobacterium Cocktail
Wójcik R. Król-Grzymała A. Tobolski D. Paritova A. García-Calvo E. Miciński J. Zwierzchowski G.
October 2025Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences
2025#26Issue 19
The ban on antibiotic growth promoters in livestock has intensified the search for effective probiotic alternatives. This study assessed the impact of a novel probiotic cocktail—comprising Lactobacillus plantarum AMT14 and AMT4, L. rhamnosus AMT15, and Bifidobacterium animalis AMT30—on the serum metabolome of lambs using an untargeted GC/MS approach. Sixteen Kamieniec lambs were divided into control and probiotic groups, with serum collected on days 0, 15, and 30. Metabolomic profiling revealed significant alterations in lipid and amino acid metabolism in the probiotic group. By day 15, 38 metabolites were upregulated, including 9,12-octadecadienoic acid, arachidonic acid, and cholesterol. On day 30, key increases included D-glucose, oleic acid, glycine, decanoic acid, and L-leucine. Multivariate analyses (PCA, PLS-DA) demonstrated clear separation between groups, and ROC analysis identified strong biomarkers with high predictive accuracy. These results suggest that probiotic supplementation can beneficially modulate host metabolism, potentially enhancing immune and physiological function in lambs. This highlights the value of multi-strain LAB-Bifidobacterium probiotics as a promising strategy for improving health and reducing antibiotic reliance in ruminant production systems.
GC/MS , lambs , probiotics , ruminants , untargeted metabolomics
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Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-718, Poland
Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-719, Poland
Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, 02-787, Poland
Department of Veterinary Sanitation, Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agrotechnical Research University, Astana, 010011, Kazakhstan
Department of Analytical Chemistry, Faculty of Chemical Sciences, Complutense University of Madrid, Madrid, 28040, Spain
Department of Sheep and Goat Breeding, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-719, Poland
Department of Microbiology and Clinical Immunology
Department of Biochemistry
Department of Large Animal Diseases and Clinic
Department of Veterinary Sanitation
Department of Analytical Chemistry
Department of Sheep and Goat Breeding
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