Nanocochleates containing N-Octylglicoside extracted Vibrio cholerae antigens elicited high vibriocidal antibodies titers after intragastric immunization in a mice model
Tamargo S.B. Bui Thanh T. Pérez M. Otero O. Oliva H.R. Falero G. Pérez J.L. Cedré M.B. Okuskhanova E. Thiruvengadam M. Shariati M.A. Sierra G.V.G.
July 2021Academic Press
Microbial Pathogenesis
2021#156
Biological detergents are used in research laboratories, to extract or solubilize proteins from cell membranes. In order to evaluate the ability to extract antigens from the bacterial cell surface of the wild Vibrio cholerae strain C7258 and study their immunogenic potential by forming proteoliposomes and cochleate and preserving their immunogenicity, the non-ionic detergent, n-Octylglucoside (n-OG), and the Zwitterionic detergent (3-cholamidopropyl dimethylammonio 1-propanesulfonate; CHAPS) were tested in concentrations between 5 and 15%. The anionic detergent sodium deoxycholate (DOC) was used as a reference. Electrophoretic, immunochemical and electron microscopy techniques have characterized the extracts and their chromatographic fractions. With CHAPS and n-OG detergents in concentrations between 5 and 15%, a higher yield was obtained in the extraction of proteins and lipopolysaccharides (LPS) and other components from the bacterial surface compared to 10% DOC. When using 10% DOC, 15% CHAPS and n-OG between 5 and 15%, stable proteoliposomes were formed, of average size between 82 and 93 nm in diameter, with known proportions of proteins, LPS and other components. In some of the concentrations, liposomes were formed with almost pure proteins. Some cholera outer membrane proteins like the 17 kDa protein, which corresponds to the mannose-sensitive hemagglutinin (MSHA), which mediates the adhesion to the brush border of the small intestine and the outer membrane protein U (OMPU) were identified with monoclonal antibodies (mAbs) and purified. The fundamental components of liposomes, proteins and LPS, retained their molecular weights, when compared with known standards and by processing programs of electrophoretic profiles and their antigenicity, without alterations due to the extraction procedure, as could be verified by immune identification techniques with monoclonal antibodies in the case of LPS, significant antigens in this pathogen. The main purpose of the present work was to show that a new anticholera vaccine formulation based on cochleates, containing selected protein and LPS fraction extracted by detergents, is able to elicit protective high titers of bactericidal antibodies after intragastric immunization in the mice model. The objective was achieved.
Bactericidal antibodies , Cochleate , Detergents , Intragastric immunization , Lipopolysaccharide , n-Octylglucoside , Outer membrane proteins , Proteoliposomes , SEM , TEM , Vibrio cholerae
Text of the article Перейти на текст статьи
Latin American School of Medicine, Havana, Cuba
Pharmacy and Foods Institute, University of Havana, Cuba
Finlay Institute for Vaccines, Havana City, Cuba
Shakarim State University of Semey, Semey, 071412, Kazakhstan
Department of Crop Science, College of Sanghuh Life Science, Konkuk University, Seoul, 05029, South Korea
Laboratory of Biological Control and Antimicrobial Resistance, Orel State University, Orel City, 302026, Russian Federation
K.G. Razumovsky Moscow State University of Technologies and Management (The First Cossack University), Moscow, Russian Federation
Kazakh Research Institute of Processing and Food Industry (Semey Branch), Kazakhstan
Group of Biotechnology and Pharmaceutical Industries of Cuba (BioCubaFarma), Havana, Cuba
Jiangxi Institute of Biological Products, JiAn, China
Latin American School of Medicine
Pharmacy and Foods Institute
Finlay Institute for Vaccines
Shakarim State University of Semey
Department of Crop Science
Laboratory of Biological Control and Antimicrobial Resistance
K.G. Razumovsky Moscow State University of Technologies and Management (The First Cossack University)
Kazakh Research Institute of Processing and Food Industry (Semey Branch)
Group of Biotechnology and Pharmaceutical Industries of Cuba (BioCubaFarma)
Jiangxi Institute of Biological Products
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026