Phylogenetic characteristics of the porcine reproductive and respiratory syndrome virus isolated from pigs in four regions of Kazakhstan
Sultanuly Z. Mambetaliyev M. Ilgekbayeva G.D. Sobiech P. Miciński J. Platt-Samoraj A. Orzechowski B. Kozhabergenov N.S. Batyrova K.I.
2025Polska Akademia Nauk
Polish Journal of Veterinary Sciences
2025#28Issue 3409 - 418 pp.
Porcine reproductive and respiratory syndrome (PRRS) causes serious reproductive and respiratory effects in pig populations worldwide. The aim of this study was to determine the seroprevalence and phylogenetic relationships of field isolates of the PRRS virus (PRRSV) in pig populations in four regions of Kazakhstan. A representative sample of 323 pigs was selected to establish the seroprevalence of PRRSV in the study area. A serological test for detecting antibodies (ELISA) was performed, and the genetic characteristics of the virus were examined using the PCR method. Virus isolation from the blood sample, MARC-145, cell cultures were used. In total, 13.93% of the tested serum samples (n=45) revealed seropositivity against PRRSV antibodies. The genetic sequencing of the isolated strains indicated the widespread circulation of the North American genotype in the Almaty region. These results were confirmed by the cytopathic effects observed in cell cultures. Molecular analysis showed that the local strains have undergone significant genetic drift from the sequenced European strains and are phylogenetically more closely related to strains originating from North American. The results of genetic analyses indicate that the North American genotype has become established in Kazakhstan. This situation requires increased use of biosecurity systems and vaccines specific for this genotype. Our results highlight the importance of continuous monitoring and an active adaptive management system in controlling the spread of PRRS in regional pig populations.
betaarterivirus suid 2 , North American genotype , phylogenetic relationships , porcine reproductive and respiratory syndrome (PRRS) , seroprevalence
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Faculty of Veterinary Medicine, Kazakh National Agrarian Research University, Abaya 8, Almaty, 050010, Kazakhstan
Research Institute for Biological Safety Problems, Laboratory for Cell Biotechnology, Guardeyskiy 15, Momyshuly, Zhambyl region, 080409, Kazakhstan
Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, Olsztyn, 10-719, Poland
Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5/150, Olsztyn, 10-719, Poland
Faculty of Veterinary Medicine, Department of Epizootiology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, Olsztyn, 10-719, Poland
Abai Kazakh National Pedagogical University, Institute of Natural Sciences and Geography, Dostyk Ave 13, Almaty, 050010, Kazakhstan
Faculty of Veterinary Medicine
Research Institute for Biological Safety Problems
Faculty of Veterinary Medicine
Faculty of Animal Bioengineering
Faculty of Veterinary Medicine
Abai Kazakh National Pedagogical University
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