Perinatal and early-life cobalt exposure impairs essential metal metabolism in immature ICR mice
Skalny A.V. Gluhcheva Y. Ajsuvakova O.P. Pavlova E. Petrova E. Rashev P. Vladov I. Shakieva R.A. Aschner M. Tinkov A.A.
March 2021Elsevier Ltd
Food and Chemical Toxicology
2021#149
The objective of the present study was to assess the impact of cobalt (Co) exposure on tissue distribution of iron (Fe), copper (Cu), manganese (Mn), and zinc (Zn), as well as serum hepcidin levels in immature mice (18, 25, 30 days). Pregnant mice were exposed to 75 mg/kg b.w. cobalt chloride (CoCl2 × 6H2O) with drinking water starting from 3 days before delivery and during lactation. At weaning (day 25) the offspring were separated and housed in individual cages with subsequent exposure to 75 mg/kg b.w. CoCl2 until 30 days postnatally. Evaluation of tissue metal levels was performed by an inductively coupled plasma-mass spectrometry (ICP-MS). Serum hepcidin level was assayed by enzyme linked immunosorbent assay (ELISA). Cobalt exposure resulted in a time- and tissue-dependent increase in Co levels in kidney, spleen, liver, muscle, erythrocytes, and serum on days 18, 25, and 30. In parallel with increasing Co levels, CoCl2 exposure resulted in a significant accumulation of Cu, Fe, Mn, and Zn in the studied tissues, with the effect being most pronounced in 25-day-old mice. Cobalt exposure significantly increased serum hepcidin levels only in day18 mice. The obtained data demonstrate that Co exposure may alter essential metal metabolism in vivo.
Cobalt , Copper , Hepcidin , Iron , Toxicity
Text of the article Перейти на текст статьи
Yaroslavl State University, Yaroslavl, Russian Federation
IM Sechenov First Moscow State Medical University, Moscow, 119146, Russian Federation
KG Razumovsky Moscow State University of Technologies and Management, Moscow, Russian Federation
Institute of Experimental Morphology, Pathology and Anthropology with Museum – Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria
Federal Research Centre of Biological Systems and Agro-technologies of the Russian Academy of Sciences, Orenburg, 460000, Russian Federation
Institute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov” – Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria
Kazakh Academy of Nutrition, Almaty, Kazakhstan
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, United States
Yaroslavl State University
IM Sechenov First Moscow State Medical University
KG Razumovsky Moscow State University of Technologies and Management
Institute of Experimental Morphology
Federal Research Centre of Biological Systems and Agro-technologies of the Russian Academy of Sciences
Institute of Biology and Immunology of Reproduction “Acad. Kiril Bratanov” – Bulgarian Academy of Sciences
Kazakh Academy of Nutrition
Department of Molecular Pharmacology
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026