In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study
Seisenov B. Duimbayev D. Kazhgaliyev N. Abdrakhmanov T. Tegza A. Abeldinov R. Burambayeva N. Temirzhanova A. Tegza I. Kemeshev Z. Zhanabayev A. Akhmetbekov N. Aisin M. Zhugunissov K. Issimov A.
August 2023Multidisciplinary Digital Publishing Institute (MDPI)
Life
2023#13Issue 8
In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population has reduced drastically over the last thirty years. The reproduction characteristics of Kazakh Whiteheaded cows were compared to Aberdeen Angus cows. Transvaginal ultrasound-guided ovum pick up sessions were carried out followed by in vitro embryo production and embryo transfer and pregnancy diagnosis. The total and viable oocytes per OPU procedure were 12.8 ± 1.18 and 8.7 ± 0.85 for the Aberdeen Angus breed, and 8.8 ± 1.04 and 6.2 ± 0.83 for the Kazakh Whiteheaded breed. Similarly, the mean number of cleaved oocytes and morula/blastocyst stage embryos produced by OPU/IVF were 4.8 ± 0.49 and 1.4 ± 0.15 for the Aberdeen Angus breed, and 2.4 ± 0.46 and 0.18 ± 0.05 for the Kazakh Whiteheaded breed (p ≤ 0.02). From fifty Kazakh Whiteheaded donor animals, 2585 oocytes were aspirated following six ovum pick up sessions. One thousand eight hundred and seventy-six (72.5%) oocytes were chosen for maturation and were further fertilized. The number of embryos cleaved was 720 (38.3% out of oocytes fertilized) on day four post-fertilization. Of these cleaved embryos, 56 (7.5%) developed into the late morula/blastocyst stage on day seven post-fertilization, averaging 1.12 embryos per donor animal. Pregnancy was detected in 12 recipients; 4 healthy calves have been born to date. The outcomes of our study have demonstrated that reproductive technologies can be applicable in preserving the endangered Kazakh Whiteheaded cattle. The findings in this report will enhance knowledge of the reproductive characteristics of endangered domestic animals and help develop sophisticated reproductive protocols for animals with unique reproductive mechanisms.
embryo , IVF , Kazakh Whiteheaded cattle
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Assyl Tulik, Republican Center for Livestock Breeding, Astana, 010000, Kazakhstan
Department of Animal Husbandary and Bioresourses, Zhangir Khan West Kazakhstan Agrarian—Technical University, Oral, 090000, Kazakhstan
Faculty of Veterinary and Animal Husbandary Technology, Saken Seifullin Kazakh Agrotechnical University, Astana, 010000, Kazakhstan
Department of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kostanay, 110000, Kazakhstan
Department of Zootechnology, Genetics and Breeding, Toraighyrov University, Pavlodar, 140000, Kazakhstan
Research Institute for Biological Safety Problems, Gvardeiskiy, 080409, Kazakhstan
Department of Biology, K. Zhubanov Aktobe Regional University, Aktobe, 030000, Kazakhstan
Assyl Tulik
Department of Animal Husbandary and Bioresourses
Faculty of Veterinary and Animal Husbandary Technology
Department of Veterinary Medicine
Department of Zootechnology
Research Institute for Biological Safety Problems
Department of Biology
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