Prebiotic Cellulose–Pullulan Matrix as a “Vehicle” for Probiotic Biofilm Delivery to the Host Large Intestine
Savitskaya I. Zhantlessova S. Kistaubayeva A. Ignatova L. Shokatayeva D. Sinyavskiy Y. Kushugulova A. Digel I.
January 2024Multidisciplinary Digital Publishing Institute (MDPI)
Polymers
2024#16Issue 1
This study describes the development of a new combined polysaccharide-matrix-based technology for the immobilization of Lactobacillus rhamnosus GG (LGG) bacteria in biofilm form. The new composition allows for delivering the bacteria to the digestive tract in a manner that improves their robustness compared with planktonic cells and released biofilm cells. Granules consisting of a polysaccharide matrix with probiotic biofilms (PMPB) with high cell density (>9 log CFU/g) were obtained by immobilization in the optimized nutrient medium. Successful probiotic loading was confirmed by fluorescence microscopy and scanning electron microscopy. The developed prebiotic polysaccharide matrix significantly enhanced LGG viability under acidic (pH 2.0) and bile salt (0.3%) stress conditions. Enzymatic extract of feces, mimicking colon fluid in terms of cellulase activity, was used to evaluate the intestinal release of probiotics. PMPB granules showed the ability to gradually release a large number of viable LGG cells in the model colon fluid. In vivo, the oral administration of PMPB granules in rats resulted in the successful release of probiotics in the colon environment. The biofilm-forming incubation method of immobilization on a complex polysaccharide matrix tested in this study has shown high efficacy and promising potential for the development of innovative biotechnologies.
bacterial cellulose , biofilms , immobilization , Lactobacillus rhamnosus GG , prebiotic
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Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Kazakh Academy of Nutrition, Almaty, 050008, Kazakhstan
Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana, 010000, Kazakhstan
Institute for Bioengineering, Aachen University of Applied Sciences, Heinrich-Mußmann-Straße 1, Jülich, D-52428, Germany
Faculty of Biology and Biotechnology
Kazakh Academy of Nutrition
Laboratory of Human Microbiome and Longevity
Institute for Bioengineering
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