Registered Influenza Viral Vector Based Brucella abortus Vaccine for Cattle in Kazakhstan: Age-Wise Safety and Efficacy Studies
Ryskeldinova S. Zinina N. Kydyrbayev Z. Yespembetov B. Kozhamkulov Y. Inkarbekov D. Assanzhanova N. Mailybayeva A. Bugybayeva D. Sarmykova M. Khairullin B. Tabynov K. Bulashev A. Aitzhanov B. Abeuov K. Sansyzbay A. Yespolov T. Renukaradhya G.J. Olsen S. Oñate A. Tabynov K.
1 July 2021Frontiers Media S.A.
Frontiers in Cellular and Infection Microbiology
2021#11
A novel influenza viral vector based Brucella abortus vaccine (Flu-BA) was introduced for use in cattle in Kazakhstan in 2019. In this study, the safety and efficacy of the vaccine was evaluated in male and female cattle at different ages, and during pregnancy as a part of its registration process. Our data demonstrated that the Flu-BA vaccine was safe after prime or booster vaccination in calves (5–7 months old male and female), heifers (15–17 months old) and cows (6–7 years old) and was not abortogenic in pregnant animals. A mild, localized granuloma was observed at the Flu-BA injection site. Vaccinated animals did not show signs of influenza infection or reduced milk production in dairy cows, and the influenza viral vector (IVV) was not recovered from nasal swabs or milk. Vaccinated animals in all age groups demonstrated increased IgG antibody responses against Brucella Omp16 and L7/L12 proteins with calves demonstrating the greatest increase in humoral responses. Following experimental challenge with B. abortus 544, vaccinates demonstrated greater protection and no signs of clinical disease, including abortion, were observed. The vaccine effectiveness against B. abortus 544 infection was 75, 60 and 60%, respectively, in calves, heifers and adult cows. Brucella were not isolated from calves of vaccinated cattle that were experimentally challenged during pregnancy. Our data suggests that the Flu-BA vaccine is safe and efficacious in cattle, including pregnant animals; and can therefore be administered to cattle of any age. © Copyright
bovine brucellosis , calves , cows , heifers , influenza viral vector , protective efficacy , registration trials , vaccine
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Infectious Disease Prevention Laboratory, Research Institute for Biological Safety Problems, Gvardeiskiy, Kazakhstan
Microbiology Laboratory, Research Institute for Biological Safety Problems, Gvardeiskiy, Kazakhstan
International Center for Vaccinology, Kazakh National Agrarian University (KazNAU), Almaty, Kazakhstan
Infectious Disease Monitoring Laboratory, Research Institute for Biological Safety Problems, Gvardeiskiy, Kazakhstan
Preclinical Research Laboratory With Vivarium, M. Aikimbayev National Research Center for Especially Dangerous Infections, Almaty, Kazakhstan
Department of Microbiology and Biotechnology, S. Seifullin Kazakh Agrotechnical University, Nur-Sultan, Kazakhstan
Department of Clinical Veterinary Medicine, Kazakh National Agrarian University (KazNAU), Almaty, Kazakhstan
Department of Biological Safety, Kazakh National Agrarian University (KazNAU), Almaty, Kazakhstan
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University (OSU), Wooster, OH, United States
Independent Researcher, McCallsburg, IA, United States
Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, University of Concepcion, Concepcion, Chile
Infectious Disease Prevention Laboratory
Microbiology Laboratory
International Center for Vaccinology
Infectious Disease Monitoring Laboratory
Preclinical Research Laboratory With Vivarium
Department of Microbiology and Biotechnology
Department of Clinical Veterinary Medicine
Department of Biological Safety
Food Animal Health Research Program
Independent Researcher
Laboratory of Molecular Immunology
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