Unlocking the potential of biofilm-forming plant growth-promoting rhizobacteria for growth and yield enhancement in wheat (Triticum aestivum L.)
Rafique M. Naveed M. Mumtaz M.Z. Niaz A. Alamri S. Siddiqui M.H. Waheed M.Q. Ali Z. Naman A. Rehman S.U. Brtnicky M. Mustafa A.
December 2024Nature Research
Scientific Reports
2024#14Issue 1
Plant growth-promoting rhizobacteria (PGPR) boost crop yields and reduce environmental pressures through biofilm formation in natural climates. Recently, biofilm-based root colonization by these microorganisms has emerged as a promising strategy for agricultural enhancement. The current work aims to characterize biofilm-forming rhizobacteria for wheat growth and yield enhancement. For this, native rhizobacteria were isolated from the wheat rhizosphere and ten isolates were characterized for plant growth promoting traits and biofilm production under axenic conditions. Among these ten isolates, five were identified as potential biofilm-producing PGPR based on in vitro assays for plant growth-promoting traits. These were further evaluated under controlled and field conditions for their impact on wheat growth and yield attributes. Surface-enhanced Raman spectroscopy analysis further indicated that the biochemical composition of the biofilm produced by the selected bacterial strains includes proteins, carbohydrates, lipids, amino acids, and nucleic acids (DNA/RNA). Inoculated plants in growth chamber resulted in larger roots, shoots, and increase in fresh biomass than controls. Similarly, significant increases in plant height (13.3, 16.7%), grain yield (29.6, 17.5%), number of tillers (18.7, 34.8%), nitrogen content (58.8, 48.1%), and phosphorus content (63.0, 51.0%) in grains were observed in both pot and field trials, respectively. The two most promising biofilm-producing isolates were identified through 16 s rRNA partial gene sequencing as Brucella sp. (BF10), Lysinibacillus macroides (BF15). Moreover, leaf pigmentation and relative water contents were significantly increased in all treated plants. Taken together, our results revealed that biofilm forming PGPR can boost crop productivity by enhancing growth and physiological responses and thus aid in sustainable agriculture.
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Soil Bacteriology Section, Agricultural Biotechnology Research Institute, AARI, Faisalabad, 38000, Pakistan
Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, 38040, Pakistan
College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China, Lahore, Pakistan
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia
Wheat Breeding Group, Plant Breeding and Genetics Division, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, 38000, Pakistan
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, 38040, Pakistan
Department of Chemistry, University of Agriculture, Faisalabad, 38040, Pakistan
Agricultural Biotechnology Research Institute, AARI, Faisalabad, 38000, Pakistan
Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, Brno, 61300, Czech Republic
Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China
Director, Programs and Projects Department, Islamic Organization for Food Security, Astana, 019900, Kazakhstan
Soil Bacteriology Section
Institute of Soil and Environmental Sciences
College of Agronomy
Department of Botany and Microbiology
Wheat Breeding Group
Department of Plant Breeding and Genetics
Department of Chemistry
Agricultural Biotechnology Research Institute
Department of Agrochemistry
Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems
Director
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