Genome-wide analysis of Glutathione peroxidase (GPX) gene family in Chickpea (Cicer arietinum L.) under salinity stress
Parveen K. Saddique M.A.B. Ali Z. Ur Rehman S. Zaib-Un-Nisa Khan Z. Waqas M. Munir M.Z. Hussain N. Muneer M.A.
10 March 2024Elsevier B.V.
Gene
2024#898
Chickpea is the second most widely grown legume in the world. Its cultivation is highly affected by saline soils. Salt stress damages its all growth stages from germination to maturity. It has a huge genetic diversity containing adaptation loci that can help produce salt-tolerant cultivars. The glutathione peroxidase (GPX) gene family plays an important role in regulating plant response to abiotic stimuli and protects cells from oxidative damage. In current research, the role of GPX genes is studied for inducing salt tolerance in chickpea. This study identifies the GPX gene family in Cicer arietinum. In response to the NaCl stress, the gene expression profiles of CaGPX3 were examined using real-time qRT-PCR. The results of phylogenetic analysis show that CaGPX genes have an evolutionary relationship with monocots, dicots, chlorophytes, and angiosperms. Gene structure analysis showed that CaGPX3, CaGPX4, and CaGPX5 have six, CaGPX2 has five, and CaGPX1 contains 9 exons. According to the Ka and Ks analysis chickpea has one pair of duplicated genes of GPX and the duplication was tandem with negative (purifying) selection Ka < Ks (<1). In-silico gene expression analysis revealed that CaGPX3 is a salt stress-responsive gene among all other five GPX members in chickpea. The qRT-PCR results showed that the CaGPX3 gene expression was co-ordinately regulated under salt stress conditions, confirming CaGPX3′s key involvement in salt tolerance.
CaGPX3 , Chickpea , Gene expression , Phylogenetic analysis , qRT-PCR , Salt stress
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Institute of Plant Breeding and Biotechnology, MNS University of Agriculture Multan, Pakistan
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan
Programs and Projects Department, Islamic Organization for Food Security, Astana, Kazakhstan
SINO-PAK Joint Research Laboratory, Institute of Plant Breeding and Biotechnology, MNS University of Agriculture Multan, Pakistan
Cotton Research Institute, Punjab, Multan, Pakistan
Pakistan Agricultural Research Council, Arid Zone Research Center, Pakistan Agricultural Research Council, Dera Ismail Khan, Pakistan
School of Environment and Energy, Peking University Shenzhen Graduate School, 2199 Lishui Rd., Shenzhen, 518055, China
Arid Zone Research Institute Bhakkar, Punjab, Pakistan
International Magnesium Institute, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China
Institute of Plant Breeding and Biotechnology
Department of Plant Breeding and Genetics
Programs and Projects Department
SINO-PAK Joint Research Laboratory
Cotton Research Institute
Pakistan Agricultural Research Council
School of Environment and Energy
Arid Zone Research Institute Bhakkar
International Magnesium Institute
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