The Assessment of Methyl Methanesulfonate Absorption by Amphipods from the Environment Using Lux-Biosensors
Novoyatlova U.S. Kudryavtseva A.A. Bazhenov S.V. Utkina A.A. Fomin V.V. Nevmyanov S.A. Zhoshibekova B.S. Fedyaeva M.A. Kolobov M.Y. Manukhov I.V.
September 2024Multidisciplinary Digital Publishing Institute (MDPI)
Biosensors
2024#14Issue 9
The ability of aquatic mesofauna representatives involved in trophic chains to sorb and accumulate toxicants is important for understanding the functioning of aquatic ecosystems and for fishing industry. This study investigated the capacity of marine amphipod Gammarus oceanicus and freshwater amphipods Eulimnogammarus vittatus and Gammarus lacustris to absorb the DNA-alkylating agent methyl methanesulfonate (MMS). The presence of alkylating agents in the environment and in the tissues of the amphipods was determined using whole-cell lux-biosensor Escherichia coli MG1655 pAlkA-lux, in which the luxCDABE genes from Photorhabdus luminescens, enabling the luminescence of the cell culture, are controlled by the PalkA promoter of DNA glycosylase. It was shown that within one day of incubation in water containing MMS at a concentration above 10 μM, the amphipods absorbed the toxicant and their tissues produce more alkylation damage to biosensor cells than the surrounding water. Concentrations of MMS above 1 mM in the environment caused the death of the amphipods before the toxicant could be significantly concentrated in their tissues. The sensitivity and the capacity to absorb MMS were found to be approximately the same for the marine amphipod G. oceanicus and the freshwater amphipods E. vittatus and G. lacustris.
absorption , alkylation , amphipod , biosensor , environmental control , methyl methanesulfonate
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Moscow Center for Advanced Studies, Moscow, 123592, Russian Federation
A.N. Severtsov Institute of Ecology and Evolution of the RAS, Moscow, 119071, Russian Federation
Youth Educational Expeditions, Dolgoprudny, 141701, Russian Federation
Department of Biology, Kazakh National Women’s Teacher Training University, Almaty, 050000, Kazakhstan
Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russian Federation
Laboratory of Microbiology, BIOTECH University, Moscow, 125080, Russian Federation
Moscow Center for Advanced Studies
A.N. Severtsov Institute of Ecology and Evolution of the RAS
Youth Educational Expeditions
Department of Biology
Faculty of Biology
Laboratory of Microbiology
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