Composition of the Microbiome of Rainbow Trout (Oncorhynchus mykiss) and Siberian Sturgeon (Acipenser baerii) and Options for its Correction with Probiotics
Kirkimbayeva Z. Sarsembayeva N. Zhylkaidar A. Sarybaeva D. Biyashev B. Akkozova A.
May-Jun, 2025Unique Scientific Publishers
International Journal of Veterinary Science
2025#14Issue 3498 - 511 pp.
The microbiome of fish plays a major role in preserving the populations health and obtaining safe, high-quality products. Antibiotic treatment in aquaculture creates problems associated with the emergence of a resistant population of microflora, which can be transmitted to humans through food, direct contact, or the environment. Thus, it is a justified and promising research objective to search for methods to increase the fish population in commercial fish farming using effective probiotic and synbiotic preparations. The study analyzes the microbiome of rainbow trout (Oncorhynchus mykiss) and Siberian sturgeon (Acipenser baerii). The composition of fish intestinal microflora is studied using microbiological analysis with determination of resistance to antibiotics and biofilm-forming ability in the identified strains and molecular genetic identification of bacterial strains using Sanger sequencing of the 16S rRNA gene with phylogenetic analysis. The fish microbiome is found to include the representatives of the genus Aeromonas, Acinetobacter, Citrobacter, Corynebacterium, Enterobacter, Escherichia, Hafnia, Pseudomonas, and Staphylococcus. In fish with signs of pathological processes, the content of opportunistic microflora is significantly increased. The isolated pure cultures of microorganisms identified as Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii, and Aeromonas sobria are resistant to six antibiotics (out of the 19 tested) and can form biofilms. In laboratory conditions, the probiotic strain Escherichia coli 39 SN shows antagonistic properties to the Aeromonas. These results can be used in the development of probiotics and synbiotics for aquaculture.
Antibiotic resistance , Aquaculture , Biofilm formation , Microbiome , Prebiotics , Synbiotics
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Department of Microbiology, Virology and Immunology, Faculty of Veterinary Science, Kazakh National Agrarian Research University, Almaty, Kazakhstan
Department of Veterinary Sanitary Examination and Hygiene, Faculty of Veterinary Science, Kazakh National Agrarian Research University, Almaty, Kazakhstan
Department of Microbiology
Department of Veterinary Sanitary Examination and Hygiene
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