Determination of Cytotoxic Compounds of Lepista personata (Fr.) Cooke by Gas Chromatography-Mass Spectrometry (GC-MS) and Chemometrics
Berdesh T. Çakir C. Çam D. Tuna K. Yeskaliyeva B. Öztürk M.
2025Taylor and Francis Ltd.
Analytical Letters
2025#58Issue 122007 - 2018 pp.
The cytotoxic activities of hexane, acetone, and methanol extracts of Lepista personata were evaluated against lung (H1299), prostate (LNCaP), colon (CaCo-2), and breast (MCF-7) cancer cell lines. The hexane extract of Lepista personata exhibited significant cytotoxic activity against CaCo-2 (EC50: 198.7 ± 4.9 µg/mL), LNCaP (EC50: 152.1 ± 3.8 µg/mL), and MCF-7 (EC50: 98.37 ± 2.5 µg/mL). Only the hexane extract exhibited cytotoxicity; thus, it was fractionated to enrich the cytotoxicity against the studied cancerous cell lines over a silica gel column. Gas chromatography-mass spectrometry (GC-MS) analyses and the cytotoxicity of all fractions were investigated. Principal component analysis (PCA) was applied to the GC-MS and cytotoxicity (EC50) results. According to the PCA, the cytotoxic fractions containing abundant benzoic acid (0.01–26.19%), cinnamic acid (0.01–5.63%), arachidic acid (0.01–17.93%), heneicosanoic acid (0.01–14.57%), 1-monolinolein (0.01–26.34%), stigmasterol (0.01–10.15%) and ergosterol (0.01–17.10%) were clustered. Moreover, the chemical composition comparison of hexane extract and its fractions, when evaluated together with PCA, revealed that the bioactive compounds detected as benzoic acid, cinnamic acid, azelaic acid, 1-monolinolein, stigmasterol, and ergosterol, which were concentrated in fractions 3, 6, 7, and 9, were responsible for the cytotoxic activity. When used with bioactivity and chemometric analysis, GC-MS of the fractions identified the bioactive compounds.
Cytotoxic activity , edible mushroom , gas chromatography-mass spectrometry (GC-MS) , lepista personata , principal component analysis (PCA)
Text of the article Перейти на текст статьи
Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Almaty, Kazakhstan
Faculty of Science, Department of Chemistry, Mugla Sitki Kocman University, Mugla, Turkey
Food Analyses Application and Research Center, Mugla Sitki Kocman University, Mugla, Turkey
Faculty of Chemistry and Chemical Technology
Faculty of Science
Food Analyses Application and Research Center
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026