First Report of Zoonotic Cryptosporidium parvum Subtype IIaA15G2R1 in Dogs in Türkiye

Ayan A. Celik B.A. Celik O.Y. Akyildiz G. Kilinc O.O. Ayan O.O. Oguz F.E. Goz Y. Yuksek N. Yilmaz A.B. Myrzhiyeva A. Akhmetzhanova A. Uslu U.
2024 University of Agriculture

Pakistan Veterinary Journal
2024 #44 Issue 4 1263 - 1268 pp.

Cryptosporidium (C.) is an opportunistic protozoan causing gastrointestinal illness in both humans and animals, leading to acute or chronic diarrhea and even death. The study aimed to investigate the prevalence and subtyping of Cryptosporidium spp. in shelter dogs in Van province, Türkiye. For microscopic identification of this parasite, a total of 300 fecal samples were collected and stained with Kinyouns acid-fast method. For molecular analysis, the positive samples were subjected to DNA extraction and SSU rRNA gene of Cryptosporidium spp. was amplified using nested PCR. The microscopic examination revealed a 4.67% prevalence of Cryptosporidium spp. Sequence analysis indicated all samples were positive to C. parvum. In addition, GP60 gene was also amplified and C. parvum subtypes IIaA15G2R1 was confirmed by analyzing the obtained sequences. All the sequences of SSU rRNA and GP60 were deposited in GenBank. To our knowledge, Cryptosporidium parvum subtypes IIaA15G2R1 have been reported first time in dogs in Türkiye. It is recommended to implement control strategies by awareness campaign, preventing stray dogs from freely entering public areas, and proper disposal of dog feces.

Cryptosporidium parvum , Dog , Molecular characterization van , Subtype iiaa15g2r1 , Türkiye

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Department of Genetics, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Türkiye
Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye
Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye
Department of Basic Health Sciences, Health Sciences Faculty, Marmara University, Istanbul, Türkiye
Ozalp Vocational School, Van Yuzuncu Yil University, Van, Türkiye
Department of Parasitology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Türkiye
Tuzluca Vocational School, Igdir University, Igdir, Türkiye
Department of Nutrition and Dietetics, Faculty of Health Sciences, Van Yuzuncu Yil University, Van, Türkiye
Department of Internal Medicine, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Türkiye
Faculty of Health, Van Yuzuncu Yil University, Van, Türkiye
Veterinary Sciences, Kazakh National Agrarian Research University, Kazakhstan
Department of Veterinary, Faculty of Agrarian, Shakarim State University of Semey, Kazakhstan
Department of Parasitology, Faculty of Medicine, University of Istanbul Medeniyet, Istanbul, Türkiye

Department of Genetics
Department of Parasitology
Department of Internal Medicine
Department of Basic Health Sciences
Ozalp Vocational School
Department of Parasitology
Tuzluca Vocational School
Department of Nutrition and Dietetics
Department of Internal Medicine
Faculty of Health
Veterinary Sciences
Department of Veterinary
Department of Parasitology

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