The ability of Lactobacillus helveticus -13 (Lh-13) Isolate Isolated from Lactic Acid Products to form A biofilm by Applying Modern Microscopy Methods
Amirkhanova Z. Akhmetova S. Kozhakhmetov S. Kushugulova A. Bodeeva R. Ahvlediani L. Turmukhambetova A.
March 2023Hashemite University
Jordan Journal of Biological Sciences
2023#16Issue 163 - 71 pp.
This article presents the study of the morpho-cultural properties and biofilm formation of lactobacilli isolated from lactic acid products (irimchik, suzbe, cheese, ashygan kozhe) produced in different districts of the Karaganda region. The species of the isolated lactic acid bacillus isolates were identified by using a MALDI-TOF mass spectrometer. The evaluation of the resistance of the isolates to various stress factors, such as the ability to survive at low acidic pH values and in the presence of bile, made it possible to identify some promising isolates – Lactobacillus helveticus -13 (Lh-13) and Lactiplantibacillus plantarum – 5 (Lpl-5), for studying antimicrobial and biofilm-forming activity. The antimicrobial activity of Lh-13, Lpl-5 was closely studied. They have a certain level of antibacterial and fungicidal activities, which indicates their pronounced inhibition against indicator microorganisms. Using a laboratory robot, TecanEVolizer100 (Tecan), the optical density of Lh-13, Lpl-5 was studied in the wells of polystyrene plates, reflecting the intensity of biofilm formation along their surface. Using laser capture microdissection, the images of cell morphology in biofilm of Lh-13 isolates included in the matrix were obtained. The atomic force microscopy made it possible to study the two and three-dimensional morphological images of biofilm-forming isolates of Lh-13.
antimicrobial activity , atomic force microscopy , biofilm , lactobacilli , laser capture microdissection , mass spectrometry (MALDI-TOF MS)
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Buketov Karaganda University, University Street,28, Karaganda, Kazakhstan
Karaganda medical university, Gogol Street,40, Karaganda, Kazakhstan
Laboratory of Human Microbiome and Longevity “National Laboratory Astana” Nazarbayev University, Kabanbay Batyr Ave.,53, Astana, Kazakhstan
Batumi International University, Fridon khalvashi street,237, Batumi, Georgia
Buketov Karaganda University
Karaganda medical university
Laboratory of Human Microbiome and Longevity “National Laboratory Astana” Nazarbayev University
Batumi International University
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