Endophytic Bacteria and Fungi Associated with Polygonum cuspidatum in the Russian Far East

Aleynova O.A. Ananev A.A. Nityagovsky N.N. Suprun A.R. Zhanbyrshina N.Z. Beresh A.A. Ogneva Z.V. Tyunin A.P. Kiselev K.V.
September 2024 Multidisciplinary Digital Publishing Institute (MDPI)

Plants
2024 #13 Issue 18

Polygonum cuspidatum, alternatively known as Fallopia japonica or Reynoutria japonica, is a perennial herb belonging to the Polygonaceae family. Commonly called Japanese knotweed or Asian knotweed, this plant is native to East Asia, particularly in regions such as Korea, China, and Japan. It has successfully adapted to a wide range of habitats, resulting in it being listed as a pest and invasive species in several countries in North America and Europe. This study focuses on analysing the composition of the bacterial and fungal endophytic communities associated with Japanese knotweed growing in the Russian Far East, employing next-generation sequencing (NGS) and a cultivation-based method (microbiological sowing). The NGS analysis showed that the dominant classes of endophytic bacteria were Alphaproteobacteria (28%) and Gammaproteobacteria (28%), Actinobacteria (20%), Bacteroidia (15%), and Bacilli (4%), and fungal classes were Agaricomycetes (40%), Dothideomycetes (24%), Leotiomycetes (10%), Tremellomycetes (9%), Pezizomycetes (5%), Sordariomycetes (3%), and Exobasidiomycetes (3%). The most common genera of endophytic bacteria were Burkholderia-Caballeronia-Parabukholderia, Sphingomonas, Hydrotalea, Methylobacterium-Metylorubrum, Cutibacterium, and Comamonadaceae, and genera of fungal endophytes were Marasmius, Tuber, Microcyclosporella, Schizothyrium, Alternaria, Parastagonospora, Vishniacozyma, and Cladosporium. The present data showed that the roots, leaves, and stems of P. cuspidatum have a greater number and diversity of endophytic bacteria and fungi compared to the flowers and seeds. Thus, the biodiversity of endophytic bacteria and fungi of P. cuspidatum was described and analysed for the first time in this study.

Asian knotweed , endophyte diversity , Fallopia japonica , Japanese knotweed , microbiome , next-generation sequencing , Reynoutria japonica

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Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russian Federation
The Department of Agriculture and Plant Growing, S. Seifullin Kazakh Agrotechnical Research University, Astana, 010011, Kazakhstan
Institute of the World Ocean, Far Eastern Federal University, Vladivostok, 690090, Russian Federation

Laboratory of Biotechnology
The Department of Agriculture and Plant Growing
Institute of the World Ocean

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