Genome-wide analysis and prediction of chloroplast and mitochondrial RNA editing sites of AGC gene family in cotton (Gossypium hirsutum L.) for abiotic stress tolerance
Ahmad F. Abdullah M. Khan Z. Stępień P. Rehman S.U. Akram U. Rahman M.H.U. Ali Z. Ahmad D. Gulzar R.M.A. Ali M.A. Salama E.A.A.
December 2024BioMed Central Ltd
BMC Plant Biology
2024#24Issue 1
Background: Cotton is one of the topmost fiber crops throughout the globe. During the last decade, abrupt changes in the climate resulted in drought, heat, and salinity. These stresses have seriously affected cotton production and significant losses all over the textile industry. The GhAGC kinase, a subfamily of AGC group and member of serine/threonine (Ser/Thr) protein kinases group and is highly conserved among eukaryotic organisms. The AGC kinases are compulsory elements of cell development, metabolic processes, and cell death in mammalian systems. The investigation of RNA editing sites within the organelle genomes of multicellular vascular plants, such as Gossypium hirsutum holds significant importance in understanding the regulation of gene expression at the post-transcriptional level. Methods: In present work, we characterized twenty-eight GhAGC genes in cotton and constructed phylogenetic tree using nine different species from the most primitive to the most recent. Results: In sequence logos analyses, highly conserved amino acid residues were found in G. hirsutum, G. arboretum, G. raimondii and A. thaliana. The occurrence of cis-acting growth and stress-related elements in the promoter regions of GhAGCs highlight the significance of these factors in plant development and abiotic stress tolerance. Ka/Ks levels demonstrated that purifying selection pressure resulting from segmental events was applied to GhAGC with little functional divergence. We focused on identifying RNA editing sites in G. hirsutum organelles, specifically in the chloroplast and mitochondria, across all 28 AGC genes. Conclusion: The positive role of GhAGCs was explored by quantifying the expression in the plant tissues under abiotic stress. These findings help in understanding the role of GhAGC genes under abiotic stresses which may further be used in cotton breeding for the development of climate smart varieties in abruptly changing climate.
Evolution , Gene expression , Genome-wide characterization , GhAGC , RNA editing sites
Text of the article Перейти на текст статьи
Sino-Pak Joint Research Laboratory, Institute of Plant Breeding and Biotechnology, MNS University of Agriculture, Punjab, Multan, 60000, Pakistan
Institute of Crop Science, Plant Precision Breeding Academy, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, College of Agriculture and Biotechnology, Zhejiang University, Zhejiang, Hangzhou, China
Institute of Soil Science, Plant Nutrition and Environmental Protection, Wroclaw University of Environmental and Life Sciences, ul. Grunwaldzka 53, Wroclaw, 50-357, Poland
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, 38000, Pakistan
Programs and Projects Department, Islamic Organization for Food Security, Astana, Kazakhstan
Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China
Laboratory of molecular biology of plant disease resistance, institute of Biotechnology, college of agriculture and biotechnology, Zhejiang university, Hangzhou, China
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 11451, Riyadh, Saudi Arabia
Agricultural Botany Department (Genetics), Faculty of Agriculture Saba Basha, Alexandria University, Alexandria, 21531, Egypt
Sino-Pak Joint Research Laboratory
Institute of Crop Science
Institute of Soil Science
Department of Plant Breeding and Genetics
Programs and Projects Department
Institute of Nuclear Agricultural Sciences
Laboratory of molecular biology of plant disease resistance
Department of Botany and Microbiology
Agricultural Botany Department (Genetics)
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026