Lacticaseibacillus paracasei subsp. paracasei 2LB: Identification of Genes to Assess the Safety and Probiotic Potential of the Strain
Abitayeva G. Kurmangali D. Baikonys T. Bekshin Z.
October 2025Multidisciplinary Digital Publishing Institute (MDPI)
Foods
2025#14Issue 19
In this study, we conducted a whole-genome analysis of the Lacticaseibacillus paracasei subsp. paracasei 2LB isolated from Kazakh traditional fermented milk (koumiss) to identify genes associated with the safety and probiotic potential of the strain. A comparative genomic analysis of the core and pan-genome of L. paracasei 2LB was performed. Functional annotation revealed the presence of genes putatively involved in metabolism, genetic information processing, and cellular processes. In terms of safety parameters, the stability of its genetic material, the absence of the ability to synthesize virulence factors, and genes responsible for antibiotic resistance were characterized. Also, in vitro studies of the L. paracasei 2LB strain showed resistance to stress factors and antimicrobial activity, and the presence of coding sequences encoding adhesion factors, bacteriocins, bile salts, pH, cold and heat shock, and osmotic stress was observed through genomic analysis. These results indicate that the L. paracasei 2LB strain is a potential probiotic candidate and demonstrate that whole-genome analysis is a useful method for assessing the quality and safety of probiotics.
bacteriocins , carbohydrate-active enzymes , Lactocaseibacillus paracasei subsp. paracasei , probiotic potential , probiotic related multiple genes , safety and stability assessment , whole genome sequencing
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Republican Collection of Microorganisms LLP, Astana, 010000, Kazakhstan
Republican Collection of Microorganisms LLP
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