Qualitative and Quantitative Determination of α-santonin in the Aboveground parts of plants of the Caprifoliaceae Juss. Family
Zhunussova M.A. Ivasenko S.A. Suleimen Y. Sarsenbekova A.Z. Abdullabekova R.M.
2025Research Journal of Pharmacy and Technology
Research Journal of Pharmacy and Technology
2025#18Issue 1239 - 244 pp.
Currently, obtaining medicines from wild plants is relevant since natural substances and medicines based on medicinal plants have a number of undeniable advantages over synthetic medicines. Some advantages include low toxicity and a wide range of therapeutic effects, as well as a low probability of side effects and complications, except in rare cases, because of their long-term use. Studies of the chemical composition and biological activity of isolated components from wild plants have revealed their practical use in medicine and revealed new opportunities for the development of new medicines based on renewable plant resources. α-Santonin was first discovered in plants of the Caprifoliaceae Juss. family. This article presents qualitative and quantitative analysis of the content of α-santonin in the aboveground parts of Scabiosa ochroleuca L., Lomelosia isetensis (L.) Soják and Dipsacus strigosus Willd. CO2-extracts, alcohol and an aqueous extracts from CO2-meal (waste from the production of carbon dioxide extract) of Scabiosa ochroleuca L.; CO2-extracts of Lomelosia isetensis (L.) Soják, Dipsacus strigosus Willd. ex Roem. & Schult. by GC/MS, HPLC, IR-spectroscopy and UV-spectrophotometry. On the chromatograms of the tested samples of the carbon dioxide extracts of the herb scabiosa pale yellow, iset scabiosa, yellow-flowered teasel, at 101.694, 101.489 and 101.43 minutes, a peak with M/z 246 appears which corresponds to the molecular ion of sesquiterpene lactone α-santonin. In the IR spectrum the presence of absorption bands in the area 1751.37,88.64 cm-1; 1735.94,73.84 cm-1, 1728.23, 65.7 indicates the presence of carbonyl lactone ring (C=O γ-lactone), and in the field 1465.91,94.33 cm-1; 1465.91,82.32 cm-1 exocyclic methylene group linked to the carbonyl γ-lactone. The ultraviolet spectrum of a sample of carbon dioxide extract from the herb Scabiosa ochroleuca L. in the region of 190 nm to 400 nm, it has an absorption maximum at a wavelength of 235 nm, which is characteristic of the exocyclic methylene group conjugated to the y-lactone carbonyl. Preparative high-performance liquid chromatography (HPLC) revealed that, the retention time of α-santonin was 12.00±0.3 min, which confirmed the presence of α-santonin in the studied extracts by comparing the retention time of the peak of α-santonin in the standard sample.
Caprifoliaceae Juss , CO2-extract , Qualitative Analysis , quantitative analysis , waste-free Technologies , α-santonin
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School of Pharmacy of the Karaganda Medical University, Karaganda, Kazakhstan
School of Pharmacy of the Karaganda Medical University, 40 Gogol Str, Karaganda, 100008, Kazakhstan
Department of Chemistry, Chemical Technology and Ecology of the Kazakh University of Technology and Business, building 37 A., Kayim Mukhamedkhanov str, Astana, Kazakhstan
Departments of Physical and Analytical Chemistry of the Academician Ye.A. Buketov Karaganda State University, 28 Universitetskaya str, Karaganda, 100024, Kazakhstan
School of Pharmacy of the Karaganda Medical University
School of Pharmacy of the Karaganda Medical University
Department of Chemistry
Departments of Physical and Analytical Chemistry of the Academician Ye.A. Buketov Karaganda State University
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