Development and Evaluation of a Live Attenuated Egg-Based Camelpox Vaccine
Zhugunissov K. Kilibayev S. Mambetaliyev M. Zakarya K. Kassenov M. Abduraimov Y. Bulatov Y. Azanbekova M. Absatova Z. Abeuov K. Nurgaziev R. Renukaradhya G.J. Tabynov K.
16 August 2021Frontiers Media S.A.
Frontiers in Veterinary Science
2021#8
Camelpox is an infectious viral disease of camels reported in all the camel-breeding areas of Africa, north of the equator, the Middle East and Asia. It causes huge economic loss to the camel industry. We developed a live camelpox virus vaccine candidate using an attenuated strain and evaluated its safety, immunogenicity and protective efficacy in camels. The attenuated virus strain was generated from the camelpox wild-type strain M-96 by 40 consecutive passages on the chorioallantoic membrane of 11-day-old embryonated chicken eggs, henceforth called KM-40 strain. Reversion to virulence of the KM-40 strain was evaluated in camels by three serial passages, confirmed its inability to revert to virulence and its overdose administration was also found safe. Studies of immunogenicity and protective efficacy of the candidate vaccine KM-40 strain in camels was carried out using the dose of 5 x 104.0 EID50. Our data showed complete protection against the challenge infection using the virulent wild-type camelpox virus strain M-96 (dose of 105.0 EID50) which was evaluated at 1, 3, 6 and 12 months post vaccination. In summary, our candidate live attenuated egg-based camelpox vaccine strain KM-40 was found safe, protective, and thus has the potential to use safely in field conditions. © Copyright
attenuation , camel , camelpox virus , immunogenicity , protective efficacy , safety , vaccine
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Laboratory Collection of Microorganisms, Research Institute for Biological Safety Problems, Gvardeiskiy, Kazakhstan
Testing Laboratory Control of Technology and Biological Products, Research Institute for Biological Safety Problems, Gvardeiskiy, Kazakhstan
Laboratory Cultivation of Microorganisms, Research Institute for Biological Safety Problems, Gvardeiskiy, Kazakhstan
Laboratory for Diagnostics of Infectious Diseases, Research Institute for Biological Safety Problems, Gvardeiskiy, Kazakhstan
Faculty of Veterinary Medicine and Biotechnology, Kyrgyz National Agrarian University Named After K.I. Skryabin, Bishkek, Kyrgyzstan
Department of Animal Sciences, Center for Food Animal Health, College of Food Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH, United States
International Center for Vaccinology, Kazakh National Agrarian University, Almaty, Kazakhstan
Preclinical Research Laboratory With Vivarium, M. Aikimbayev National Scientific Center for Especially Dangerous Infections, Almaty, Kazakhstan
Laboratory Collection of Microorganisms
Testing Laboratory Control of Technology and Biological Products
Laboratory Cultivation of Microorganisms
Laboratory for Diagnostics of Infectious Diseases
Faculty of Veterinary Medicine and Biotechnology
Department of Animal Sciences
International Center for Vaccinology
Preclinical Research Laboratory With Vivarium
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