COMPARATIVE STUDY OF THE GENOTOXICITY OF 9-AMINOACRIDINE AND 8-METHOXYPSORALEN USING A BACTERIAL BIOSENSOR
Zhoshibekova B. Abilev S. Sartayeva A.
April 2025Society for the Advancement of Breeding Researches in Asia and Oceania
Sabrao Journal of Breeding and Genetics
2025#57Issue 2658 - 667 pp.
A comparative study based on the genotoxicity of 9-aminoacridine (9-AA) and 8-methoxypsoralen (8-MOP) transpired using a lux biosensor E. coli MG1655 (pColD-lux), having a recombinant plasmid with a lux operon under a gene promoter colD. The colD (cda) gene is part of the E. coli SOS regulon that also ensures DNA repair and cell resistance to DNA damage. The Gen colD (cda) includes SOS-regulon E. coli. The lux operon performs a reporter function characterizing the SOS response to DNA damage. The considered genotoxicity of 9-AA and activated UVA (λ = 365 nm) 8-MOP came from manipulating the luminescence intensity of the biosensor. The 8-MOP induction in bacteria of the SOS response depended on concentrations of UVA and 8-MOP. With higher doses of UVA, a 25-fold decrease emerged in the survival of bacterial cells (from 2х108 to 8х106 КОЕ), while an increase in the intensity of the SOS response by 675 times for 106 cells was evident in viable cells. The DNA-damaging and lethal effect of 8-MOP in bacteria relied on the concentrations of UVA and 8-MOP.
8-methoxypsoralen , 9-aminoacridine , diadducts , E. coli , gene promoter ColD , lux-biosensor , monoadducts , SOS response , UV irradiation
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Shymkent university, Shymkent, Kazakhstan
Vavilov Institute of General Genetics of Russian Academic Sciences, Moscow, Russian Federation
Department of Biology, Kazakh National Womens Teachers Training University, Almaty, Kazakhstan
Shymkent university
Vavilov Institute of General Genetics of Russian Academic Sciences
Department of Biology
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