Role of Gold Nanoparticles in Inhibiting Amyloid Formation and Reducing Vascular Dementia Risk

Zholdasbekov A. AbdulHussein A.H. Hashim A.M. Ahmed H.H. Hani M.M. Shnishil A.T. Khudair S.A. Muedii Z.A.-H.R. Egamberdievich S.S. Gafurovich K.O. Abdurashidovich U.A.
2023 University of Kashan

Journal of Nanostructures
2023 #13 Issue 3 703 - 709 pp.

Lysozyme amyloid aggregation is associated with vascular dementia and other disorders. However, conventional therapies are limited by the blood-brain barrier. Gold nanoparticles (AuNPs) could modulate amyloid aggregation and provide a novel therapeutic approach. We investigated the effect of AuNPs on lysozyme amyloid formation of Hen Egg White Lysozyme (HEWL). We incubated lysozyme (3 mg/ml) in 60 mM glycine buffer (pH 2.8) at 61oC with gentle shaking to induce amyloid formation. We used various techniques to assess the impact of AuNPs (5-50 μg/ml) on lysozyme amyloid accumulation. We performed independent t-test and SPSS 23.0 software for data analysis. AuNPs inhibited lysozyme amyloid aggregation in a concentration-dependent manner. The lowest concentration (5 μg/ml) was the most effective, as it significantly increased the lag phase and decreased the growth phase of amyloid formation, and also reduced the cytotoxicity of amyloid aggregates on cell viability. Our results suggest that AuNPs act as nano-chaperones and prevent lysozyme amyloid fibril formation, and thus they may have therapeutic potential for vascular dementia treatment.

Amyloid formation , Gold nanoparticles , Protein aggregation , Vascular dementia

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M. Auezov South Kazakhstan university, Shymkent, Kazakhstan
Department of Pharmaceutics, College of Pharmacy, University of Al-Ameed, Karbala, Iraq
Department of Pharmacy, Mazaya university college, Iraq
Department of Pharmacy, Al-Noor University College, Nineveh, Iraq
Department of Medical Instrumentation Engineering Techniques, Imam Ja’afar Al-Sadiq University, Iraq
Department of Optical Techniques, Al-Hadi University College, Baghdad, 10011, Iraq
Department of Medical Laboratories Technology, AL-Nisour University College, Baghdad, Iraq
College of Medical Technology, Medical Lab techniques, National University of Science and Technology, Dhi Qar, Iraq
Tashkent State University of Economics, Uzbekistan
Tashkent State Technical University, Tashkent, Uzbekistan

M. Auezov South Kazakhstan university
Department of Pharmaceutics
Department of Pharmacy
Department of Pharmacy
Department of Medical Instrumentation Engineering Techniques
Department of Optical Techniques
Department of Medical Laboratories Technology
College of Medical Technology
Tashkent State University of Economics
Tashkent State Technical University

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