Identification and characterization of bluetongue virus in Culicoides spp. and clinically healthy livestock in southeastern Kazakhstan
Zhigailov A.V. Perfilyeva Y.V. Maltseva E.R. Ostapchuk Y.O. Cherusheva A.S. Naizabayeva D.A. Nizkorodova A.S. Berdygulova Z.A. Mashzhan A.S. Bissenbay A.O. Kuatbekova S.A. Koshemetov Z.K. Abdolla N. Skiba Y.A. Mamadaliyev S.M.
1 November 2022Elsevier Ltd
Comparative Immunology, Microbiology and Infectious Diseases
2022#90-91
Objective: Bluetongue is an arthropod-borne disease of ruminants. Here, we investigated the seroprevalence of bluetongue virus (BTV) in livestock and performed the first genetic characterization of BTV isolated from sheep and Culicoides midges in the southeastern region of Kazakhstan. Methods: Blood samples were collected from 1241 asymptomatic livestock. In addition, 497 Culicoides midges were collected. Samples were analyzed for specific anti-BTV antibodies and BTV RNA by ELISA and conventional RT-PCR, respectively. Results: The overall seroprevalence of BTV antibodies was shown to be 4.3 % (46/1079) in small ruminant and 1 % (1/82) in cattle. Anti-BTV antibodies were not detected in camels (0/80). The minimum infection rate of BTV in Culicoides was shown to be 0.24 %. Seg-2 and Seg-10 sequence analysis demonstrated that all isolates belonged to the ‘western’ topotype of the BTV-9 strain. Conclusion: The present data confirm circulation of BTV in southeastern Kazakhstan.
Bluetongue , Culicoides , Kazakhstan , Livestock , Serotype , Virus
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Almaty Branch of the National Center for Biotechnology, Almaty, 050054, Kazakhstan
M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, 050012, Kazakhstan
Tethys Scientific Society, Almaty, 050063, Kazakhstan
Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Research Institute for Biological Safety Problems, Gvardeyskiy, Korday District, Zhambyl Region, 080409, Kazakhstan
Almaty Branch of the National Center for Biotechnology
M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry
Tethys Scientific Society
Al-Farabi Kazakh National University
Research Institute for Biological Safety Problems
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