Multidirectional Characterization of Phytochemical Profile and Health-Promoting Effects of Ziziphora bungeana Juz. Extracts
Zhaparkulova K. Karaubayeva A. Sakipova Z. Biernasiuk A. Gaweł-Bęben K. Laskowski T. Kusniyeva A. Omargali A. Bekezhanova T. Ibragimova L. Ibadullayeva G. Jakiyanov A. Czech K. Tastambek K. Głowniak K. Malm A. Kukula-Koch W.
December 2022MDPI
Molecules
2022#27Issue 24
Ziziphora species (Lamiaceae) have been used in traditional medicine as sedatives, antiseptics, carminatives, or expectorants. Despite their common applications in phytotherapy, there is still lack of evidence about the composition of their extracts and its impact on biological properties of the plants. The aim of this study was to evaluate the content of Ziziphora bungeana, a less studied species growing in Kazakhstan, using HPLC-ESI-QTOF-MS/MS instrumentation and to determine its antimicrobial, antioxidant, and cytotoxic activity together with inhibitory properties against tyrosinase and toxicity in erythrocyte lysis assay. Extracts from Z. bungeana were found to be sources of flavonoids, phenolic acids, organic acids, and terpenes that determined their antiradical activity. The minimum inhibitory concentrations of extracts were lower for Gram-positive bacteria (1.25–10 mg/mL) than for Gram-negative bacteria and fungi (5–20 mg/mL). The EC50 value calculated for antiradical activity ranged between 15.00 ± 1.06 µg/mL and 13.21 ± 3.24 µg/mL for ABTS and DPPH assays, respectively. Z. bungeana extracts were found to decrease the activity of tyrosinase by 50% (at 200 µg/mL) similarly to kojic acid and were slightly cytotoxic for human melanoma A375 cell line (at 200 µg/mL) with no effect on HaCaT keratinocytes. In the end, Z. bungeana did not reveal toxic effects in hemolytic assay as compared to the positive control Triton X-100. The performed tests show potential application of the plant in the treatment of infectious diseases, disorders caused by free radicals, and skin problems.
antimicrobial activity , antioxidant potential , cytotoxicity , erythrocyte lysis , flavonoids , HPLC-MS , Lamiaceae , tyrosinase inhibition , Ziziphora
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School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty, 050012, Kazakhstan
Chair and Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Lublin, 1, Chodzki str., Lublin, 20-093, Poland
Department of Cosmetology, University of Information Technology and Management in Rzeszów, 2 Sucharskiego str., Rzeszów, 35-225, Poland
Department of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Faculty of Chemistry, Gdańsk University of Technology, Gabriela Narutowicza Str. 11/12, Gdańsk, 80-233, Poland
Edinburgh Dental Institute, The University of Edinburgh, Edinburgh, EH8 9YL, United Kingdom
Department of Fundamental Medicine, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Department of Chemical and Biochemical Engineering, Geology and Oil-Gas Business Institute Named after K. Turyssov, Institute of Chemical and Biological Technologies, Satbayev University, Almaty, 050043, Kazakhstan
Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, 161200, Kazakhstan
Department of Pharmacognosy, Medical University of Lublin, 1, Chodźki str, Lublin, 20-093, Poland
School of Pharmacy
Chair and Department of Pharmaceutical Microbiology
Department of Cosmetology
Department of Pharmaceutical Technology and Biochemistry and BioTechMed Centre
Edinburgh Dental Institute
Department of Fundamental Medicine
Department of Chemical and Biochemical Engineering
Ecology Research Institute
Department of Pharmacognosy
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