Memory T-cell dynamics following immunization with a polyvalent salmonellosis vaccine in farm animals


Zhanabayev S.D. Biyashev B.K.
July 2024University of Mosul - College of Veterinary Medicine

Iraqi Journal of Veterinary Sciences
2024#38Issue 3653 - 661 pp.

The topic of studying the pool of memory T-cells during immunization with vaccines is relatively understudied, but this is no less important. The research aims to investigate the bodys accumulation and storage time of immune memory cells. The study used the following methods: gradient centrifugation method, flow cytofluorometry method, direct immunofluorescence reaction, four color compositions method, cluster analysis, and methods of calculating the arithmetic mean and its standard error. The study examined rabbits at 1.5 months of age. The study of quantitative changes in memory T-cells and their phenotypes showed an increase in central and effector CD4+ and CD8+ memory T-cells. Also, quantitative differences in memory cell phenotypes were observed in primary immunized animals compared to booster immunization. Most frequently, specific CD4+ T-cells, which produce cytokines, could be seen in the lymphocytes of the own lamina of the muscularis ileum and jejunum. A significant difference in the notional number of cytokine-producing characteristics between the group not subjected to the experiment and the immunized animals was dominated by CD4+ T-cells in the intestine and lymph nodes. This study showed essential factors in the development of immune cell memory and will be useful both for farmers and for the development of the topic of memory in immunization.

CD markers , Cytokine , Lymphocytes , Salmonella typhimurium

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Department of Microbiology, Virology and Immunology, Kazakh National Agrarian Research University, Almaty, Kazakhstan

Department of Microbiology

10 лет помогаем публиковать статьи Международный издатель

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