Exploring the complex interplay of Blastocystis, morbid obesity, and bariatric surgery on gut microbial dynamics
Özdemir A. Sarzhanov F. Doğruman-Al F. Gündoğdu A. Nalbantoğlu U. Yozgat A. Yıldız B.D. Büyüktuncer Z.
March 2025Academic Press
Microbial Pathogenesis
2025#200
This study examines Blastocystis dynamics in 15 individuals undergoing sleeve gastrectomy. Molecular detection involved DNA extraction, RT-PCR, and sequencing, while 16S rRNA sequencing via Illumina MiSeq analyzed the intestinal microbiome. Statistical analysis through SPSS considered a significance level of p < 0.05. Postoperative colonization of Blastocystis was observed in previously negative individuals, revealing subtypes and shifts in microbial taxa. Blastocystis-positive participants post-bariatric surgery showed a significant increase in Lachnospira, alongside higher abundances of Bacteroides, Oscillospira, Barnesiellaceae, and Rikenellaceae, with reduced Lactobacillus levels compared to Blastocystis-negative individuals. Collective analysis highlighted higher Clostridiales and RF32 in Blastocystis-positive post-surgery individuals, coupled with decreased Enterobacteriaceae. The study challenges previous notions, suggesting a complex interplay between Blastocystis, morbid obesity, and bariatric surgery. Despite limitations, including small sample sizes and absent gut microbiome diversity comparisons, this pioneering research calls for further investigation into the intricate mechanisms and implications for metabolic health. This study is registered at clinicaltrials.gov (NCT05085769).
Bariatric surgery , Blastocystis , Gastrointestinal microbiome , Obesity
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Department of Nutrition and Dietetics, Faculty of Health Sciences, Hacettepe University, Ankara, Turkey
Faculty of Medicine, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, Kazakhstan
Division of Medical Parasitology, Department of Medical Microbiology, School of Medicine, Gazi University, Ankara, Turkey
Department of Microbiology and Clinical Microbiology, School of Medicine, Erciyes University, Kayseri, Turkey
Department of Metagenomics, Genome and Stem Cell Center, Erciyes University, Kayseri, Turkey
Department of Research and Development, ENBIOSIS Biotechnology, Istanbul, Turkey
Department of Computer Engineering, School of Engineering, Erciyes University, Kayseri, Turkey
Department of Bioinformatics, Genome and Stem Cell Center, Erciyes University, Kayseri, Turkey
Department of Gastroenterology, Ankara Numune Research and Education Hospital, Ankara, Turkey
Department of Gastroenterology, Ankara Research and Education Hospital, Ankara, Turkey
Department of General Surgery, Ankara Numune Research and Education Hospital, Ankara, Turkey
University of Health Sciences, Ankara Bilkent City Hospital, Ankara, Turkey
Department of Nutrition and Dietetics
Faculty of Medicine
Division of Medical Parasitology
Department of Microbiology and Clinical Microbiology
Department of Metagenomics
Department of Research and Development
Department of Computer Engineering
Department of Bioinformatics
Department of Gastroenterology
Department of Gastroenterology
Department of General Surgery
University of Health Sciences
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