Anticancer activity of Avicennia marina leaves on breast, ovarian and cervical cancer cell lines: In vitro and In silico analyses
Zare A. Afshar A. Zare M. Khoradmehr A. Anvari M. Safarzoda Sharoffidin R. Mohebbi G. Khatami M. Mussin N.M. Mahdipour M. Daneshi A. Mahmudpour M. Nabipour I. Tamadon A.
October 2025Elsevier B.V.
South African Journal of Botany
2025#1851 - 15 pp.
This study investigates the anticancer potential of ethanol and ethyl acetate extracts from Avicennia marina leaves against breast (MCF-7), ovarian (OVCAR3), and cervical (HeLa) cancer cell lines through in vitro and in silico approaches. Phytochemical analyses revealed high phenolic and flavonoid contents, with ethanol extracts showing greater yields. In vitro assays demonstrated that ethanol extracts significantly inhibited MCF-7 proliferation via S-phase cell cycle arrest, while ethyl acetate extracts induced apoptosis in HeLa and OVCAR3 cells. Western blot analysis confirmed upregulated pro-apoptotic proteins (BAX, cleaved caspases-1, -3, -7) and downregulated anti-apoptotic proteins (BCL-2, pro-caspases). In silico molecular docking and dynamics simulations identified key compounds, including alpha amyrin, stigmasterol, and vitamin E, with strong binding affinities to apoptosis-related receptors (e.g., TNFR1, PPARγ, Adenosine A1). Bioavailability assays indicated favorable lipophilicity but poor aqueous solubility, suggesting the need for advanced delivery systems. These findings highlight A. marina as a promising source of anticancer compounds, warranting further in vivo and formulation studies.
Anticancer , Apoptosis , Avicennia marina , Caspase , Cell cycle arrest , Mangrove
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Drug Discovery and Development Industry, School of Pharmacy, Taipei Medical University, Taipei, Taiwan
PerciaVista R&D Co, Shiraz, Iran
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Pharmaceutical Technology, Avicenna Tajik State Medical University, Dushanbe, Tajikistan
General Surgery, West-Kazakhstan Medical University named after Marat Ospanov, Aktobe, Kazakhstan
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Natural Sciences, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Drug Discovery and Development Industry
PerciaVista R&D Co
The Persian Gulf Marine Biotechnology Research Center
Department of Biology and Anatomical Sciences
Department of Pharmaceutical Technology
General Surgery
Stem Cell Research Center
Department of Applied Cell Sciences
The Persian Gulf Tropical Medicine Research Center
Department of Natural Sciences
Stem Cells Technology Research Center
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