Rapid detection of carcinoembryonic antigen by means of an electrochemical aptasensor
Yunussova N. Tilegen M. Pham T.T. Kanayeva D.
17 May 2024Elsevier Inc.
iScience
2024#27Issue 5
Carcinoembryonic antigen (CEA) is a critical biomarker for identifying colon cancer. This work presents an electrochemical impedance spectroscopy (EIS) based aptasensor for detecting CEA, utilizing a single-stranded DNA (ssDNA) aptamer previously selected and characterized by our research group. The surface of an interdigitated gold electrode (IDE) was successfully functionalized with an 18-HEG-modified aptamer sequence. The developed aptasensor demonstrated high specificity and sensitivity with detection limits of 2.4 pg/mL and 3.8 pg/mL for CEA in buffer and human serum samples, respectively. The optimal incubation time for the target protein was 20 min, and EIS measurements took less than 3 min. Atomic force microscopy (AFM) micrographs supported the EIS data, demonstrating a change in IDE surface roughness after each modification step, confirming the successful capture of the target. The potential of this developed EIS aptasensor in detecting CEA in complex samples holds promise.
Applied sciences , Bio-electrochemistry , Sensor system
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Ph.D. program in Life Sciences, Department of Biology, School of Sciences and Humanities, Nazarbayev University, 53 Kabanbay batyr Avenue, Astana, 010000, Kazakhstan
M.Sc. program in Molecular Medicine, School of Medicine, Nazarbayev University, 5/1 Kerey-Zhanibek Khandar St, Astana, 010000, Kazakhstan
Department of Biology, School of Sciences and Humanities, Nazarbayev University, 53 Kabanbay batyr Avenue, Astana, 010000, Kazakhstan
Ph.D. program in Life Sciences
M.Sc. program in Molecular Medicine
Department of Biology
10 лет помогаем публиковать статьи Международный издатель
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