Bone regeneration capacity of adipose tissue-derived stem cells in rabbit periodontitis-induced bone defect model
Yermukhanova G. Rusanov V. Bayakhmetova A. Menchisheva Y. Ksetaeva G. Yermukhanova N.
2021Termedia Publishing House Ltd.
Journal of Stomatology
2021#74Issue 4227 - 235 pp.
Introduction: Periodontitis is one of the most common oral infectious disease. Adipose tissue-derived mesenchymal stem cells (AD-MSCs) have become widely studied for their possibilities for regeneration of periodontal bone defect. However, bone regeneration efficiency of AD-MSCs combined with transplantation materials in periodontal bone defects remains unclear. Objectives: The purpose of the study was to compare the effects of two repair methods with and without application of AD-MSCs in a rabbits periodontal defect model. Material and methods: Experimental periodontitis was initiated in eighteen rabbits. Animals were divided into two groups, including Col-HA only (group 1) and Col-HA + AD-MSCs (group 2), and implanted in rabbit periodontitis-induced bone defect. To evaluate difference in these two groups, we used histological analysis of the samples and X-ray. For comparison of grey-level frequency in X-ray samples of the two groups, Otsus method was applied. Results: Post-operative wounds in group 2 healed three days earlier than in group 1, starting from a formation of fibrous tissue, cartilage, and osteoid tissue, and ending with a formation of full-plate bone tissue. X-ray evaluations showed that more intensive reparative regeneration in post-operative bone cavity was observed in animals of group 2. The 90th day samples with AD-MSCs presented more differentiated gray-level than the 90th day samples without AD-MSCs. Conclusions: The results of the study suggest that the use of transplantation material (Col-HA) with AD-MSCs has positive effects, contributing to the improvement of reparative regeneration of bone tissue in the periodontal bone defect area.
Adipose tissue-derived mesenchymal stem cells , Bone regeneration , Experimental periodontitis , Periodontal surgery
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Department of Pediatric Dentistry, S.D. Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan
Department of Surgical Dentistry, S.D. Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan
Department of Therapeutic Dentistry, S.D. Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan
Department of Pediatric Dentistry
Department of Surgical Dentistry
Department of Therapeutic Dentistry
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