Insulin Conformation Changes in Hybrid Alginate–Gelatin Hydrogel Particles
Yerlan G.Y. Shen M. Tyussyupova B.B. Tazhibayeva S.M. Musabekov K. Takhistov P.
March 2024Multidisciplinary Digital Publishing Institute (MDPI)
Molecules
2024#29Issue 6
There is a strong need to develop an insulin delivery system suitable for oral administration and preserving natural (α-helix) insulin conformation. In this work, we fabricated alginate–gelatin hydrogel beads for insulin encapsulation. Altering matrix composition and crosslinking agents has resulted in various surface morphologies and internal spatial organization. The structures of the insulin-loaded matrices were studied using optical and field emission electronic microscopy. We use FTIR spectroscopy to identify insulin conformation changes as affected by the hydrogel matrices. It was found that blended alginate–gelatin matrices demonstrate better encapsulation efficiency and stronger swelling resistance to a simulated gastric environment than sodium alginate beads crosslinked with the CaCl2. FTIR measurements reveal conformation changes in insulin. It is also confirmed that in the presence of gelatin, the process of insulin fibrinogenesis ceases due to intermolecular interaction with the gelatin. Performed molecular modeling shows that dipole–dipole interactions are the dominating mechanism that determines insulin behavior within the fabricated matrix.
alginate , gelatin , hydrogel particles , insulin , insulin fibrinogenesis
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Department of Analytical, Colloid Chemistry and Technology of Rare Elements, Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, 71 Al-Farabi Ave., Almaty, 050040, Kazakhstan
Department of Food Science, The State University of New Jersey, 65 Dudley Road, New Brunswick, 08901, NJ, United States
Petroleum Engineering Institute, Kazakh-British Technical University, Tole bi Street, 59, Almaty, 050040, Kazakhstan
Department of Analytical
Department of Food Science
Petroleum Engineering Institute
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