Epigenetic Modification of HepG2 Cells by Modulating DNA (cytosine-5)-methyltransferase 1 (DNMT1) and Ten-eleven Translocation Methylcytosine Dioxygenase 1 (TET1) Expression using Persian Shallot Extract
Yarahmadi Z. Sadeghi A. Mohammadian F. Roustaei F. Hajizadeh M. Mirzaei M. Swann J. Hosseiniara R. Mahmoodi M.
2023Bentham Science Publishers
Current Chemical Biology
2023#17Issue 3196 - 201 pp.
Background: Various forms of epigenetic modification, including DNA methylation, contribute to hepatocellular carcinoma (HCC) dissemination, invasion, and metastasis. The Persian shallot (Allium hirtifolium Bioss.) is reported to have antibacterial, antifungal, antioxidant, and anti-carcinogenic properties. Aims: In the present study, we examined the expression of DNA (cytosine-5)-methyltransferase 1 (DNMT1) and ten-eleven translocation methylcytosine dioxygenase 1 (TET1) at the mRNA level in HepG2 cells following treatment with Persian shallot extract. Method: Ethanolic extracts of Persian shallot were prepared and dried at 80°C and 50°C for 20 and 30 minutes, respectively. Different concentrations of dried shallot extract over the range of 0-250 µg/ml were prepared. HepG2 cells were cultured and the cytotoxicity of each extract concentration was measured using an MTT assay. The gene expression in treated and untreated cells was assessed by Real-time polymerase chain reaction (RT-PCR). Results: The half maximal inhibitory concentration (IC50) was determined to be 149 µg/ml using an MTT assay. A concentration of 175 μg/ml was found to reduce the expression of DNMT1 in the treated group compared to the control group (P<0.001). Furthermore, the TET1 mRNA of HepG2 cells was down-regulated significantly after treatment with 100 and 1000 μg/ml of Persian shallot extract (P<0.05). These doses reduced the viability of the samples by 60% or higher. Conclusion: This study provides evidence for the potential use of Persian shallot extract as a supplementary herbal agent for the treatment of HCC. The concentrations of extract used in this study are near or above the level required for toxicity, and as such, further study is warranted.
DNA (cytosine-5)-methyltransferase 1 (DNMT1) , gene expression , HepG2 cells , neoplasm , Persian shallot , ten-eleven translocation methylcytosine dioxygenase 1 (TET1)
Text of the article Перейти на текст статьи
Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Department of Immunology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Science, Rafsanjan, Iran
Department of Biological Sciences, Lehigh University, 111 Research Drive, Bethlehem, 18015, PA, United States
Higher School of Medicine, Faculty of Medicine and Health Care, Al-Farabi Kazakh National University, Almaty, Kazakhstan
Department of Clinical Biochemistry, Afzalipoor Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Department of Clinical Biochemistry
Department of Immunology
Molecular Medicine Research Center
Department of Biological Sciences
Higher School of Medicine
Department of Clinical Biochemistry
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026