A novel IgG-Fc-Fused multiepitope vaccine against Brucella: robust immunogenicity
Wu A. Zhang Y. Liu C. Zhumanov K. He T. Yan K. Li H. Fu S. Li X. Zhang W. Meng C. Zhang C. Sheng J. Ma Z. Xu M. Zhang J. Yi J. Wang Y.
December 2025BioMed Central Ltd
Microbial Cell Factories
2025#24Issue 1
Brucellosis is one of the most common zoonotic diseases caused by Brucella spp. However, there is currently no Brucella vaccine available for humans. Although some attenuated live vaccines have been approved for animals, their protective efficacy is suboptimal. In previous studies, we utilized an epitope- and structure-based vaccinology platform to identify the immunodominant epitopes of Brucella antigens OMP19, OMP16, OMP25, and L7/L12, and constructed the multi-epitope vaccine MEV-Fc against Brucella. In this study, OMP19, OMP16, OMP25, and L7/L12, and MEV-Fc was expressed and purified via an Escherichia coli expression system, which validated that MEV-Fc possesses high immunological efficacy and exerts a significant protective effect in BALB/c mice within the Brucella infection model. MEV-Fc enhanced Th1 and Th2 immune responses and strongly induced the production of the pro-inflammatory cytokine IFN-γ. Furthermore, MEV-Fc protected mice against Brucella infection compared to control group (PBS). In conclusion, our results provide new insights and data support for the development of human Brucella vaccines.
Brucella , Multiple epitopes , Vaccines immune response
Text of the article Перейти на текст статьи
College of Animal Science and Technology, Shihezi University, Xinjiang, Shihezi, 832003, China
Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region, Tongren University, Tongren, Guizhou, 554300, China
School of Medicine, Shihezi University, Xinjiang, Shihezi, 832003, China
Kazakh National Agrarian University, Almaty, 050010, Kazakhstan
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China
Tiankang Biopharmaceutical Co., Ltd, Xinjiang, Urumqi, 830032, China
College of Animal Science and Technology
Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region
School of Medicine
Kazakh National Agrarian University
Jiangsu Key Laboratory of Zoonosis
Tiankang Biopharmaceutical Co.
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026