Electrochemical immunosensor based on broad-spectrum antibodies for detection of organophosphorus pesticide residues in fruits and vegetables
Wang X. Han C. Li D. Zhou Z. Shi M. Kozykan S. Wang W.
December 2025Elsevier B.V.
Applied Food Research
2025#5Issue 2
Diethoxythiophosphate organophosphorus pesticides are widely used in agricultural production. However, excessive pesticide use leads to the presence of pesticide residues in agricultural products, thereby affecting their safety and posing a threat to consumer health. Traditional pesticide detection equipment is characterized by high personnel requirements and is expensive and time-consuming to use, which is not conducive to the rapid detection of pesticide residues in practical applications. Although the existing electrochemical immunosensors are able to provide efficient, rapid, and portable detection of pesticide residues, they can only recognize certain residue types and cannot realize simultaneous detection of multiple residues. Therefore, an electrochemical immunosensor was developed based on PAIN/Co3O4 as the substrate material to specifically identify diethoxyphosphorothioate organophosphorus pesticides by employing broad-spectrum organophosphorus antibodies as the identification element. Detection was carried out based on the specific recognition element of each pesticide in order to achieve simultaneous detection and rapid screening of a variety of diethoxyphosphorothioate organophosphorus residues. Copyright
Broad-spectrum antibodies , Electrochemical immunosensors , Nanomaterials , Organophosphorus pesticides
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Xinjiang Vocational University of Technology, Tumushuke, China
College of Food Science and Engineering, Tarim University, Alear, China
Production & Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang, Alar, China
Central University of Nationalities, School of Information Engineering, Beijing, China
Key Laboratory of Tarim Oasis Agriculture, Ministry of Education, Tarim University, Alear, China
Kazakh National Agrarian Research University, Abay 8, Almaty, Kazakhstan
Modern Agricultural Engineering Key Laboratory at Universities of Education Department of Xinjiang Uygur Autonomous Region, Alar, China
Xinjiang Vocational University of Technology
College of Food Science and Engineering
Production & Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang
Central University of Nationalities
Key Laboratory of Tarim Oasis Agriculture
Kazakh National Agrarian Research University
Modern Agricultural Engineering Key Laboratory at Universities of Education Department of Xinjiang Uygur Autonomous Region
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