Development of a time-resolved immunochromatographic test strip for rapid and quantitative determination of GFAP in serum
Wang Y. Yu Z. Ning Z. Li M. Li W. Zhong Y. Chen H. Zhang X. Tang X. Cheng X. Li L. Aigul A. Zan J.
June 2024Springer
Microchimica Acta
2024#191Issue 6
Glial fibrillary acidic protein (GFAP) in serum has been shown as a biomarker of traumatic brain injury (TBI) which is a significant global public health concern. Accurate and rapid detection of serum GFAP is critical for TBI diagnosis. In this study, a time-resolved fluorescence immunochromatographic test strip (TRFIS) was proposed for the quantitative detection of serum GFAP. This TRFIS possessed excellent linearity ranging from 0.05 to 2.5 ng/mL for the detection of serum GFAP and displayed good linearity (Y = 598723X + 797198, R2 = 0.99), with the lowest detection limit of 16 pg/mL. This TRFIS allowed for quantitative detection of serum GFAP within 15 min and showed high specificity. The intra-batch coefficient of variation (CV) and the inter-batch CV were both < 4.0%. Additionally, this TRFIS was applied to detect GFAP in the serum samples from healthy donors and patients with cerebral hemorrhage, and the results of TRFIS could efficiently discern the patients with cerebral hemorrhage from the healthy donors. Our developed TRFIS has the characteristics of high sensitivity, high accuracy, and a wide linear range and is suitable for rapid and quantitative determination of serum GFAP on-site. Graphical abstract: (Figure presented.)
Fluorescence test strip , Glial fibrillary acidic protein , Traumatic brain injury
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School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangdong, Guangzhou, 510006, China
The Second Clinical College, Guangzhou University of Chinese Medicine, Guangdong, Guangzhou, China
The Jockey Club School of Public Health and Primary Care, Faculty of Medicine, The Chinese University of Hong Kong, SAR, Hong Kong
Department of Neurology, Hubei Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Hubei University of Traditional Chinese Medicine, Hubei, Wuhan, China
China-Uzbekistan Institute of Biomedical Industry Technology, Guangdong, Guangzhou, China
Research Institute of Preventive Medicine named Academician E. Dalenov, Astana Medical University, Astana, Kazakhstan
Guangdong Provincial Key Laboratory of Research on Emergency in TCM, Guangdong, Guangzhou, China
School of Biomedical and Pharmaceutical Sciences
The Second Clinical College
The Jockey Club School of Public Health and Primary Care
Department of Neurology
China-Uzbekistan Institute of Biomedical Industry Technology
Research Institute of Preventive Medicine named Academician E. Dalenov
Guangdong Provincial Key Laboratory of Research on Emergency in TCM
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