Biochemical and molecular genetic identification of the bacterial pathogen – Aeromonas bestiarum from a diseased Siberian sturgeon (Acipenser baerii)
Utegenova K.S. Bakiyev S.S. Mambetova D.S. Kauysbekov A.Zh. Bissenbaev A.K.
25 December 2024al-Farabi Kazakh State National University
International Journal of Biology and Chemistry
2024#17Issue 237 - 44 pp.
The article presents the results of isolation and identification of the bacterium Aeromonas bestiarum from diseased individuals of Siberian sturgeon (Acipenser baerii) reared in recirculating aquaculture system (RAS). As a result of biochemical studies, the isolated strain A. bestiarum AB002 is characterized as: a Gram-negative, non-motile oxidase-positive bacillus capable of growing in a wide temperature range from 13 to 42 ºC. In addition, strain AB002 is characterized by the hydrolysis of gelatin and esculin, forming H2 S and indole, and exhibits arginine dihydrolase activity. The analysis of bacterial resistance to antibiotics revealed that strain AB002 is resistant to multiple groups of antibiotics, including Penicillins (Oxacillin, Penicillin G, Ampicillin, Amoxicillin); Cephalosporins (Cefazolin); Macrolides (Erythromycin); Lincomycins (Lincomycin); Rifamycins (Rifampicin); Coumarins (Novobiocin). The analysis of virulence factors revealed that the pathogenic strain A. bestiarum AB002 is characterized by the presence of 6 virulence genes out of 10 studied, among which lipase (pla), cytotonic enterotoxin (alt), serine protease (ahe2), DNAase (nucl), cholesterol acyltransferase (gcaT), aerolysin (aerA) were identified.
16S rRNA gene , Acipenser baerii , Aeromonas bestiarum , biochemical characteristics , gyrB gene
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Department of Biophysics, Biomedicine and Neuroscience, Al-Farabi Kazakh National University, Almaty, Kazakhstan
Scientific Research Institute of Biology and Biotechnology Problems, Almaty, Kazakhstan
Makhambet Utemisov West Kazakhstan University, Uralsk, Kazakhstan
Department of Biodiversity and Bioresources, Al-Farabi Kazakh National University, Almaty, Kazakhstan
Department of Molecular Biology and Genetics, Al-Farabi Kazakh National University, Almaty, Kazakhstan
Department of Molecular Biology and Genetics, Scientific Research Institute of Biology and Biotechnology Problems, Al-Farabi Kazakh National University, Almaty, Kazakhstan
Department of Biophysics
Scientific Research Institute of Biology and Biotechnology Problems
Makhambet Utemisov West Kazakhstan University
Department of Biodiversity and Bioresources
Department of Molecular Biology and Genetics
Department of Molecular Biology and Genetics
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