Malignant ascites promote adhesion of ovarian cancer cells to peritoneal mesothelium and fibroblasts
Uruski P. Mikuła-Pietrasik J. Pakuła M. Budkiewicz S. Drzewiecki M. Gaiday A.N. Wierzowiecka M. Naumowicz E. Moszyński R. Tykarski A. Ksiażek K.
2 April 2021MDPI
International Journal of Molecular Sciences
2021#22Issue 8
Although malignant ascites (MAs) are known to contribute to various aspects of ovarian cancer progression, knowledge regarding their role in the adhesion of cancer cells to normal peritoneal cells is incomplete. Here, we compared the effect of MAs and benign ascites (BAs) on the adhesion of A2780 and OVCAR-3 cancer cells to omentum-derived peritoneal mesothelial cells (PMCs) and peritoneal fibroblasts (PFBs). The results showed that MAs stimulated the adhesion of A2780 and OVCAR-3 cells to PMCs and PFBs more efficiently than did BAs, and the strongest binding occurred when both cancer and normal cells were exposed to the fluid. Intervention studies showed that MAs-driven adhesion of A2780 cells to PMCs/PFBs depends on the presence of TGF-β1 and HGF, whereas binding of OVCAR-3 cells was mediated by TGF-β1, GRO-1, and IGF-1. Moreover, MAs upregulated α5β1 integrin expression on PFBs but not on PMCs or cancer cells, vimentin expression in all cells tested, and ICAM-1 only in cancer cells. When integrin-linked kinase was neutralized in PMCs or PFBs, cancer cell adhesion to PMCs and PFBs decreased. Collectively, our report shows that MAs may contribute to the early stages of ovarian cancer metastasis by modulating the proadhesive interplay between normal and cancer cells.
Cancer cell adhesion , Malignant ascites , Ovarian cancer , Peritoneal metastases
Text of the article Перейти на текст статьи
Department of Hypertensiology, Poznan University of Medical Sciences, Długa 1/2 Str., Poznan, 61-848, Poland
Department of Pathophysiology of Ageing and Civilization Diseases, Poznan University of Medical Sciences, Długa 1/2 Str., Poznan, 61-848, Poland
Department of Obstetrics and Gynecology, West Kazakhstan Marat Ospanov Medical University, 50B 12th microdistrict apt.21, Aktobe, 030008, Kazakhstan
General Surgery Ward, Medical Centre HCP, 28 czerwca 1956 r. 223/229 Str., Poznan, 61-485, Poland
Division of Gynecological Surgery, Poznan University of Medical Sciences, Polna 33 Str, Poznan, 60-535, Poland
Department of Hypertensiology
Department of Pathophysiology of Ageing and Civilization Diseases
Department of Obstetrics and Gynecology
General Surgery Ward
Division of Gynecological Surgery
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026