First Molecular Identification of Dirofilaria repens Found in a Wolf Heart in Kazakhstan
Uakhit R.S. Lider L.A. Smagulova A.M. Leontyev S.V. Abdrakhmanov S.K. Kiyan V.S.
2021Nexus Academic Publishers
Advances in Animal and Veterinary Sciences
2021#9Issue 91342 - 1346 pp.
The clinical manifestations of dirofilariasis are usually different and depend on the species: D. immitis is located on the right side of the heart and pulmonary artery. On the other hand, D. repens is localized in the subcutaneous tissue. According to the literature, dirofilariasis is a filaroid nematode present in animals and humans, which is mainly transmitted by mosquitoes. This is a rare case of finding helminths in the heart cavity of a wolf and raises questions about its distribution area in Kazakhstan. To determine the genotype of Dirofilaria spp. found in the heart of a wild wolf, identification was carried out by PCR using the species-specific primer SSU rRNA, as a result of which amplicons of 875 bp were obtained. The results of the study showed that the nucleotide sequence of the studied species was identified as D. repens, and this sequence was deposited in the NCBI GenBank database (accession number MT877205.1). The research results revealed the imperfection of the differential identification of Dirofilaria spp parasites only by their morphological structure and confirm the need to use molecular identification methods.
Dirofilaria repens , Heart , Molecular identification , SSU gene , Wolf
Text of the article Перейти на текст статьи
Research Platform of Agricultural Biotechnology
Department of Veterinary Medicine
Department of Veterinary Sanitation, Saken Seifullin Kazakh Agrotechnical University, Nur-Sultan, 010011, Kazakhstan
Facility of biology and technology, Department of biology, Bioresources and Aquaculture, Novosibirsk State Agrarian University
Research Platform of Agricultural Biotechnology
Department of Veterinary Medicine
Department of Veterinary Sanitation
Facility of biology and technology
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026