Chromatographic purification technology optimisation of immunoglobulin G (IgG) from horse serum for animal chlamydia diagnostics
Оптимізація технології хроматографічного очищення імуноглобуліну G (IgG) із сироватки коней для діагностики хламідіозу тварин
Tyrsynbayev N. Akhmetsadykov N. Krykbayev Y. Khussainov D. Mendybayeva A.
2024Polissia National University
Scientific Horizons
2024#27Issue 1020 - 30 pp.
The study aimed to develop and evaluate an antibody quality improvement method to improve the accuracy and efficiency of chlamydia diagnosis in horses. The study was conducted in Kazakhstan and included 100 horses of the Kazakh breed infected with chlamydia, which was divided into two groups: experimental and control. In the experimental group, affinity chromatography was used to purify immunoglobulin G (IgG), which allowed for a 95% purity of the antibodies. The control group used a traditional diagnostic method without preliminary purification, which ensured IgG purity of only 60-65%. The data showed that the purified antibodies demonstrated improved diagnostic performance, including an increase in sensitivity of up to 92% and specificity of up to 95%. The purified antibodies provided effective binding to chlamydial antigens even at low concentrations (0.2cμg/ml), which is 2.5 times better than in the control group. The time to obtain a stable diagnostic signal was reduced by 33% and amounted to 20 minutes in the experimental group versus 30 minutes in the control group. The frequency of false-positive results in the experimental group was reduced to 5% and false-negative results to 4%, which significantly increases the overall reliability of diagnostics. The purified antibodies retained their activity for 12 months, demonstrating high stability and durability. These results highlighted the importance of using chromatographic purification to improve the quality of antibodies used for diagnostic purposes and offer a reliable approach for the accurate detection of chlamydia as well as other infectious diseases in animals. The introduction of such methods can significantly improve the efficiency of veterinary diagnostics and contribute to more timely and adequate treatment of animals
antibody stability , Kazakh breed , parasitology , predictive value , specificity
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