The Model of Acute Obstructive Pyelonephritis for Studying Bacterial Translocation of E. coli from Gastroenteric Tract
Turgunov Y. Shakeyev K. Sharapatov Y. Lavrinenko A. Pronkin E.
1 January 2022Scientific Foundation SPIROSKI
Open Access Macedonian Journal of Medical Sciences
2022#10232 - 235 pp.
BACKGROUND: The role of intestinal microflora translocation in the development of obstructive pyelonephritis has not been sufficiently studied. The urgency to develop a new model of acute obstructive pyelonephritis is due to the search for characteristics that are able to meet the criteria for reproducibility of microbial translocation from the intestine, the reversibility of the stages of the inflammatory process with further observation in the dynamics of development. AIM: The aim of the given research is to develop a model of acute obstructive pyelonephritis to study the pathogenetic role of bacterial translocation of Escherichia coli (hereinafter E. coli) from the gastrointestinal tract (GIT). METHODS: Twenty outbred male rabbits aged 3 months and weighing 3.0 ± 0.5 kg were used for the research. All the animals were randomly divided into two groups: Experimental (n = 10) and control (n = 10). In the experimental group, obstructive pyelonephritis was modeled by ligating the external opening of the urethra and injecting an antibiotic-resistant E. coli strain into the GIT using enteric capsules. In the control group, the strain was administered in the same way, but without forming a model of obstructive pyelonephritis. The animals were withdrawn from the experiment on the 3rd day by air embolism under general anesthesia. In both groups, the sizes of the kidney, pelvis, ureter, and the number of leukocytes in urine were assessed. RESULTS: In the experimental group, there was an increase in the size of the kidney, pelvis, as well as ureter with some pronounced leukocyturia observed, which indicates the development of obstructive pyelonephritis. In the control group, only one animal had leukocyturia. The statistically significant differences were revealed between the groups in all studied parameters. CONCLUSION: The results of this research demonstrated that the proposed model provided an opportunity to study the role of intestinal translocation of microorganisms in the development of acute obstructive pyelonephritis.
Acute pyelonephritis , Bacteria , Intestinal translocation , Obstruction
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Department of Surgical Diseases, Non-commercial Joint-Stock Company, Medical University of Karaganda, Karaganda, Kazakhstan
Collective Use Laboratory, Non-Commercial Joint-Stock Company, Karaganda Medical University, Karaganda, Kazakhstan
Department of Urology, Central State Medical Academy of Department of Presidential Affairs of the Russian Federation, Moscow, Russian Federation
Department of Surgical Diseases
Collective Use Laboratory
Department of Urology
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