Restoring populations of Populus pruinosa Schrenk through biotechnology
Turdiyev T. Kovalchuk I. Madenova A. Kuan A. Mikhailenko N. Kabylbekova B. Baizhumanova S. Yemesheva K. Tuigunov Z. Rakhimbayev I.
February 2025Springer
In Vitro Cellular and Developmental Biology - Plant
2025#61Issue 1207 - 218 pp.
The most effective method to combat desertification is planting artificial forests of Populus pruinosa. In the Zhetysu and Almaty regions, samples were gathered from natural stands, which P. pruinosa and P. diversifolia likely displaced. The morphological attributes of 27 samples were related to one species, but one sample was causing doubt. PCR analyses were performed using eight microsatellite markers. The genotyping results revealed that all of the 28 samples collected were of the same type of P. pruinosa. They can effectively be introduced into in vitro culture in winter when physiological dormancy has ended. Annual lignified cuttings 6 to 7 cm long with 1 to 2 buds from selected plus trees were sterilized from saprophytic microflora by bleach NaOCl (1:1) for 10 min and 0.1% HgCl2 solution for 5 min and placed on the medium. Both male and female plants were propagated in the medium that contained ½ Murashige and Skoog (MS), 0.1 mg L-1BAP; 0.02 mg L-1GA; 20 g L-1 Glucose; pH 5.7, where the multiplication rate averaged 3.8 to 4.5, and the shoot length at 5.7 to 7.0 cm in 4 to 6 wk. For the transferring regenerated plants to soil in pots, the best substrate was found as peat, humus, and sand at a ratio of 50:40:10, respectively.
Aseptic plants , Clonal micropropagation , P. pruinosa , Pot plant , Rhizogenesis
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Laboratory of Plant Microclonal Propagation, Kazakh National Agrarian Research University, Almaty, 050010, Kazakhstan
Laboratory of Germplasm Cryopreservation, Institute of Plant Biology and Biotechnology, Almaty, 050040, Kazakhstan
Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Laboratory of Plant Microclonal Propagation
Laboratory of Germplasm Cryopreservation
Department of Biotechnology
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